C57BL/6JCya-Srsf4em1/Cya
Common Name:
Srsf4-KO
Product ID:
S-KO-17533
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Srsf4-KO
Strain ID
KOCMP-57317-Srsf4-B6J-VB
Gene Name
Product ID
S-KO-17533
Gene Alias
5730499P16Rik; MNCb-2616; SRp75; Sfrs4
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srsf4em1/Cya mice (Catalog S-KO-17533) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053819
NCBI RefSeq
NM_020587.2
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Srsf4, a member of the serine-/arginine (SR)-rich protein family, is involved in both constitutive and alternative splicing of pre-mRNA, and plays a crucial role in RNA metabolism [2]. As an essential component of spliceosomes, it also participates in processes like spliceosome assembly, mRNA export, and nonsense-mediated mRNA decay, which are vital for maintaining cellular and tissue homeostasis [5].
Srsf4 has been linked to multiple diseases and biological processes. In glioma, its high expression is associated with histological grade, IDH1 status, 1p/19q codeletion, molecular subtype, tumor recurrence, and poor prognosis. Srsf4 promotes temozolomide (TMZ) resistance by positively regulating MDC1 and accelerating double-strand break repair, and targeting it can improve chemosensitivity [1]. In pulmonary hypertension, Srsf4 upregulates the expression of ca-circFOXP1 by splicing exons 6 and 9 of FOXP1 pre-mRNA, which then affects pulmonary arterial smooth muscle cell proliferation through the R-loop-mediated regulation of FOXP1 and SMAD2 phosphorylation [3]. In patients with a missense variant of the Srsf4 gene, lower Srsf4 protein expression leads to mitochondrial dysfunction, presenting as leukopenia, lymphopenia, and neutropenia [4].
In conclusion, Srsf4 is a key regulator in RNA splicing and metabolism, with its dysregulation contributing to various diseases such as glioma, pulmonary hypertension, and bone marrow failure. Understanding Srsf4 through functional studies, including potential gene knockout models, provides insights into disease mechanisms and may offer new therapeutic targets.
References:
1. Sun, Yi, Liu, Xingdong, Wu, Zhiqiang, Yan, Wei, You, Yongping. 2023. SRSF4 Confers Temozolomide Resistance of Glioma via Accelerating Double Strand Break Repair. In Journal of molecular neuroscience : MN, 73, 259-268. doi:10.1007/s12031-023-02115-0. https://pubmed.ncbi.nlm.nih.gov/37014544/
2. Masaki, So, Kabuto, Takafumi, Suzuki, Kenji, Kataoka, Naoyuki. 2020. Multiple nuclear localization sequences in SRSF4 protein. In Genes to cells : devoted to molecular & cellular mechanisms, 25, 327-333. doi:10.1111/gtc.12756. https://pubmed.ncbi.nlm.nih.gov/32050040/
3. Song, Xinyue, Xu, Ya, Li, Mengnan, Zheng, Xiaodong, Zhu, Daling. 2025. SRSF4-Associated ca-circFOXP1 Regulates Hypoxia-Induced PASMC Proliferation by the Formation of R Loop With Host Gene. In Arteriosclerosis, thrombosis, and vascular biology, 45, e118-e135. doi:10.1161/ATVBAHA.124.322026. https://pubmed.ncbi.nlm.nih.gov/39973750/
4. Miano, Maurizio, Bertola, Nadia, Grossi, Alice, Cappelli, Enrico, Ravera, Silvia. 2024. Impaired Mitochondrial Function and Marrow Failure in Patients Carrying a Variant of the SRSF4 Gene. In International journal of molecular sciences, 25, . doi:10.3390/ijms25042083. https://pubmed.ncbi.nlm.nih.gov/38396760/
5. Tan, Wanyan, Wang, Wei, Ma, Qingfeng. 2018. Physiological and Pathological Function of Serine/Arginine-Rich Splicing Factor 4 and Related Diseases. In BioMed research international, 2018, 3819719. doi:10.1155/2018/3819719. https://pubmed.ncbi.nlm.nih.gov/29789787/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen