C57BL/6JCya-Oas2em1/Cya
Common Name:
Oas2-KO
Product ID:
S-KO-17570
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Oas2-KO
Strain ID
KOCMP-246728-Oas2-B6J-VB
Gene Name
Product ID
S-KO-17570
Gene Alias
Oasl11
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Oas2em1/Cya mice (Catalog S-KO-17570) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000081491
NCBI RefSeq
NM_001347448.1
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Oas2, short for 2'-5'-oligoadenylate synthetase 2, is an interferon (IFN)-induced antiviral enzyme. It belongs to the OAS family, which also includes OAS1, OAS3, and OAS-like (OASL). Oas2 can generate 2'-5'-linked oligoadenylates upon activation by double-stranded RNA, which is involved in the RNA cleavage pathway and antiviral response system [6,8].
In breast cancer, high Oas2 mRNA expression is associated with favorable prognosis, potentially making it a prognostic biomarker [1]. In psoriasis, Oas2 is overexpressed in lesional skin and serum, and its expression is downregulated by biologics. Silencing Oas2 inhibits keratinocyte proliferation by regulating the cell cycle and suppressing IFN-1-induced phosphorylation of Jak1 and signal transducer and activator of transcription 1, suggesting it may be a therapeutic biomarker [2]. In SARS-CoV-2-related multisystem inflammatory syndrome in children, autosomal recessive deficiencies of Oas2 lead to excessive production of inflammatory cytokines upon viral stimulation, underlying the disease mechanism [3]. In mammary cancer, an activating mutation in Oas2 prevents pregnancy-driven metastases and enhances the effectiveness of checkpoint immunotherapy, establishing it as a therapeutic target [4]. In testicular cancer, upregulating Oas2 by piR-36249 and DHX36 inhibits cancer cell progression [5]. Oas2 also restricts intracellular Mycobacterium tuberculosis replication and enhances pro-inflammatory cytokine secretion [6], and inhibits Zika virus replication through activation of the Type Ι IFN signaling pathway [7].
In conclusion, Oas2 plays crucial roles in multiple disease conditions, including cancer, psoriasis, and infectious diseases. Its functions in these diseases range from being a prognostic biomarker, a therapeutic target, to being involved in the regulation of cell proliferation, cytokine production, and antiviral responses. Studies, especially those with potential loss-of-function models, have significantly contributed to our understanding of Oas2's role in these biological processes and disease conditions.
References:
1. Zhang, Yujie, Yu, Chaoran. 2020. Prognostic characterization of OAS1/OAS2/OAS3/OASL in breast cancer. In BMC cancer, 20, 575. doi:10.1186/s12885-020-07034-6. https://pubmed.ncbi.nlm.nih.gov/32560641/
2. Huang, Yan-Zhou, Zheng, Yu-Xin, Zhou, Yuan, Zheng, Min, Man, Xiao-Yong. 2022. OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augmenting IFN-1‒Induced Jak1‒Signal Transducer and Activator of Transcription 1 Phosphorylation in Psoriasis. In The Journal of investigative dermatology, 142, 2635-2645.e9. doi:10.1016/j.jid.2022.02.018. https://pubmed.ncbi.nlm.nih.gov/35305973/
3. Lee, Danyel, Le Pen, Jérémie, Yatim, Ahmad, Zhang, Shen-Ying, Casanova, Jean-Laurent. 2023. Inborn errors of OAS-RNase L in SARS-CoV-2-related multisystem inflammatory syndrome in children. In Science (New York, N.Y.), 379, eabo3627. doi:10.1126/science.abo3627. https://pubmed.ncbi.nlm.nih.gov/36538032/
4. Ho, Wing-Hong Jonathan, Law, Andrew M K, Masle-Farquhar, Etienne, Oakes, Samantha R, Ormandy, Christopher J. 2022. Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases. In Breast cancer research : BCR, 24, 31. doi:10.1186/s13058-022-01525-z. https://pubmed.ncbi.nlm.nih.gov/35505346/
5. Wang, Qianqian, Chen, Peize, Wang, Xiaorong, Song, Xiaoyuan, Sun, Fei. 2023. piR-36249 and DHX36 together inhibit testicular cancer cells progression by upregulating OAS2. In Non-coding RNA research, 8, 174-186. doi:10.1016/j.ncrna.2022.12.004. https://pubmed.ncbi.nlm.nih.gov/36710986/
6. Leisching, Gina, Cole, Victoria, Ali, Aus T, Baker, Bienyameen. 2019. OAS1, OAS2 and OAS3 restrict intracellular M. tb replication and enhance cytokine secretion. In International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 80S, S77-S84. doi:10.1016/j.ijid.2019.02.029. https://pubmed.ncbi.nlm.nih.gov/30822544/
7. Liao, Xinzhong, Xie, He, Li, Shilin, Yang, Chunhui, Chen, Limin. 2020. 2', 5'-Oligoadenylate Synthetase 2 (OAS2) Inhibits Zika Virus Replication through Activation of Type Ι IFN Signaling Pathway. In Viruses, 12, . doi:10.3390/v12040418. https://pubmed.ncbi.nlm.nih.gov/32276512/
8. Koul, Amit, Deo, Soumya, Booy, Evan P, Genung, Matthew, McKenna, Sean A. 2019. Impact of double-stranded RNA characteristics on the activation of human 2'-5'-oligoadenylate synthetase 2 (OAS2). In Biochemistry and cell biology = Biochimie et biologie cellulaire, 98, 70-82. doi:10.1139/bcb-2019-0060. https://pubmed.ncbi.nlm.nih.gov/30965010/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen