C57BL/6JCya-Oas3em1/Cya
Common Name:
Oas3-KO
Product ID:
S-KO-17571
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Oas3-KO
Strain ID
KOCMP-246727-Oas3-B6J-VB
Gene Name
Product ID
S-KO-17571
Gene Alias
Oasl10
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Oas3em1/Cya mice (Catalog S-KO-17571) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044833
NCBI RefSeq
NM_145226
Target Region
Exon 3
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
Oas3, short for 2'-5'-oligoadenylate synthetase 3, is an enzyme induced by interferons and belongs to the 2', 5' oligoadenylate synthase family. It activates potential RNA enzymes to degrade viral mRNA, inhibit viral protein synthesis, and promote apoptosis in virus-infected cells. Oas3 is involved in the IFN-β1b/JAK/STAT1 pathway and the OAS-RNase L antiviral pathway [2,5]. It is also associated with the tumor microenvironment, disease staging, prognosis, and treatment response in multiple cancer types [3].
In sepsis-induced acute lung injury (SALI), downregulation of E3 ligase TRIM21 leads to Oas3 deubiquitination at the K1079 site in lung epithelial cells. This increases Oas3 protein level in a proteasomal-dependent manner, promoting epithelial cell apoptosis through its downstream effector molecule, RNase L, and driving SALI [1]. In pancreatic cancer, Oas3 is upregulated by pancreatic cancer cells via the lactate/METTL3/OAS3 axis. Oas3 in macrophages is essential for M2d polarization, and its deficiency impairs M2d polarization and pro-tumor functions, enhancing the efficacy of gemcitabine and anti-PD-L1 mAb in humanized mouse models [4].
In conclusion, Oas3 is crucial in antiviral defense, apoptosis regulation, and tumor-related processes. Studies using mouse models in SALI and pancreatic cancer have revealed its role in disease development, providing potential therapeutic targets for these diseases.
References:
1. Chen, Zhenfeng, Lin, Bingqi, Yao, Xiaodan, Liu, Zhifeng, Guo, Xiaohua. 2024. OAS3 Deubiquitination Due to E3 Ligase TRIM21 Downregulation Promotes Epithelial Cell Apoptosis and Drives Sepsis-induced Acute Lung Injury. In International journal of biological sciences, 20, 5594-5607. doi:10.7150/ijbs.96089. https://pubmed.ncbi.nlm.nih.gov/39494334/
2. Zheng, Baisong, Zhou, Xiaolei, Tian, Li, Wang, Jian, Zhang, Wenyan. 2022. IFN-β1b induces OAS3 to inhibit EV71 via IFN-β1b/JAK/STAT1 pathway. In Virologica Sinica, 37, 676-684. doi:10.1016/j.virs.2022.07.013. https://pubmed.ncbi.nlm.nih.gov/35934228/
3. Li, Xin-Yu, Hou, Lei, Zhang, Lu-Yu, Wen, Ming-Zhe, Yang, Xi-Tao. 2022. OAS3 is a Co-Immune Biomarker Associated With Tumour Microenvironment, Disease Staging, Prognosis, and Treatment Response in Multiple Cancer Types. In Frontiers in cell and developmental biology, 10, 815480. doi:10.3389/fcell.2022.815480. https://pubmed.ncbi.nlm.nih.gov/35592250/
4. Zhang, Shaopeng, Xu, Ximo, Zhang, Kundong, Huang, Chen, Qiu, Zhengjun. 2024. Targeting OAS3 for reversing M2d infiltration and restoring anti-tumor immunity in pancreatic cancer. In Cancer immunology, immunotherapy : CII, 74, 37. doi:10.1007/s00262-024-03898-w. https://pubmed.ncbi.nlm.nih.gov/39738657/
5. Oh, Sunwoo, Santiago, Gisselle, Manjunath, Lavanya, Semler, Bert L, Buisson, Rémi. 2024. A CRISPR-Cas9 knockout screening identifies IRF2 as a key driver of OAS3/RNase L-mediated RNA decay during viral infection. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2412725121. doi:10.1073/pnas.2412725121. https://pubmed.ncbi.nlm.nih.gov/39475651/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen