C57BL/6JCya-Tgfbiem1/Cya
Common Name:
Tgfbi-KO
Product ID:
S-KO-17608
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tgfbi-KO
Strain ID
KOCMP-21810-Tgfbi-B6J-VA
Gene Name
Product ID
S-KO-17608
Gene Alias
68kDa; Beta-ig; big-h3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tgfbiem1/Cya mice (Catalog S-KO-17608) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045173
NCBI RefSeq
NM_009369
Target Region
Exon 4~6
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Tgfbi, also known as transforming growth factor-β-induced gene, encodes an extracellular matrix protein, TGFBIp. TGFBIp is involved in multiple biological processes. It has been associated with various pathways, and its functions include roles in cell-matrix interactions, tissue development, and repair [2]. Genetic models, such as gene knockout mouse models, are valuable for studying its functions.
In pancreatic cancer, TGFBI produced by tumor-associated macrophages (TAMs) promotes M2 polarization of macrophages, which in turn stimulates pancreatic cancer growth. Loss-of-function of TGFBI in macrophages, both in vitro (using siRNA) and in vivo (using Cre-Lox strategy in TGFBI-floxed mice), inhibited M2 polarization, reduced macrophage-mediated pancreatic cancer growth, improved anti-tumor immunity, and sensitized pancreatic cancer to chemotherapy [1]. In breast cancer, deletion of Tgfbi in a mouse model led to a decrease in cancer stem cell (CSC) content and lung metastasis. Lack of TGFBI resulted in tumor vessel normalization, improved vessel perfusion, and decreased hypoxia, factors controlling CSCs and metastasis [3].
In conclusion, Tgfbi plays crucial roles in various biological processes and disease conditions. The use of gene knockout mouse models has revealed its significance in pancreatic and breast cancer, among others. These studies provide insights into Tgfbi's functions and suggest potential therapeutic targets for these malignancies.
References:
1. Zhou, Jing, Lyu, Nan, Wang, Qiongling, Staveley-O'Carroll, Kevin F, Li, Guangfu. 2023. A novel role of TGFBI in macrophage polarization and macrophage-induced pancreatic cancer growth and therapeutic resistance. In Cancer letters, 578, 216457. doi:10.1016/j.canlet.2023.216457. https://pubmed.ncbi.nlm.nih.gov/37865162/
2. Nielsen, Nadia Sukusu, Poulsen, Ebbe Toftgaard, Lukassen, Marie V, Hjortdal, Jesper, Enghild, Jan J. 2020. Biochemical mechanisms of aggregation in TGFBI-linked corneal dystrophies. In Progress in retinal and eye research, 77, 100843. doi:10.1016/j.preteyeres.2020.100843. https://pubmed.ncbi.nlm.nih.gov/32004730/
3. Fico, Flavia, Santamaria-Martínez, Albert. 2020. TGFBI modulates tumour hypoxia and promotes breast cancer metastasis. In Molecular oncology, 14, 3198-3210. doi:10.1002/1878-0261.12828. https://pubmed.ncbi.nlm.nih.gov/33080107/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen