C57BL/6JCya-Polqem1/Cya
Common Name
Polq-KO
Product ID
S-KO-17642
Backgroud
C57BL/6JCya
Strain ID
KOCMP-77782-Polq-B6J-VB
Status
When using this mouse strain in a publication, please cite “Polq-KO Mouse (Catalog S-KO-17642) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Polq-KO
Strain ID
KOCMP-77782-Polq-B6J-VB
Gene Name
Product ID
S-KO-17642
Gene Alias
A430110D14Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 16
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000054034
NCBI RefSeq
NM_029977
Target Region
Exon 2~12
Size of Effective Region
~30.0 kb
Overview of Gene Research
POLQ, also known as DNA polymerase theta, is a key enzyme in DNA double-strand break (DSB) repair, mainly involved in the microhomology-mediated end-joining (MMEJ) pathway. It has both helicase and polymerase activities, playing a crucial role in maintaining genome stability. Genetic models, such as gene knockout in cells, are valuable for studying its functions [1,2,4,5,6].
POLQ-deficient cells accumulate post-replicative ssDNA gaps upon BRCA1/2 loss or PARP inhibitor treatment, indicating its role in post-replicative gap sealing. In HR-deficient pancreatic adenocarcinoma, POLQ knockdown is synthetically lethal with HR gene mutations, and it also activates the cGAS-STING signaling pathway, enhancing tumor immune infiltration. In gastric cancer, POLQ knockdown suppresses the stemness of cancer cells and their resistance to ferroptosis. In telomere-driven crisis models, POLQ-/-cells show unique telomere-related phenotypes and reduced chromosomal abnormalities [1,2,3,4].
In conclusion, POLQ is essential for maintaining genome stability through its role in DSB repair and post-replicative gap sealing. Studies using POLQ-deficient models have revealed its significance in cancer-related processes, such as synthetic lethality in HR-deficient cancers, regulation of cancer cell stemness and ferroptosis resistance, and influence on telomere-related genomic stability. These findings provide potential therapeutic targets for cancer treatment [1,2,3,4].
References：
1. Belan, Ondrej, Sebald, Marie, Adamowicz, Marek, Rueda, David S, Boulton, Simon J. 2022. POLQ seals post-replicative ssDNA gaps to maintain genome stability in BRCA-deficient cancer cells. In Molecular cell, 82, 4664-4680.e9. doi:10.1016/j.molcel.2022.11.008. https://pubmed.ncbi.nlm.nih.gov/36455556/
2. Oh, Grace, Wang, Annie, Wang, Lidong, Sfeir, Agnel, Simeone, Diane M. 2023. POLQ inhibition elicits an immune response in homologous recombination-deficient pancreatic adenocarcinoma via cGAS/STING signaling. In The Journal of clinical investigation, 133, . doi:10.1172/JCI165934. https://pubmed.ncbi.nlm.nih.gov/36976649/
3. Peng, Yanmei, Zheng, Wenbo, Chen, Yuehong, Li, Guoxin, Yu, Jiang. 2024. POLQ inhibition attenuates the stemness and ferroptosis resistance in gastric cancer cells via downregulation of dihydroorotate dehydrogenase. In Cell death & disease, 15, 248. doi:10.1038/s41419-024-06618-5. https://pubmed.ncbi.nlm.nih.gov/38575587/
4. Liddiard, Kate, Aston-Evans, Alys N, Cleal, Kez, Hendrickson, Eric A, Baird, Duncan M. 2022. POLQ suppresses genome instability and alterations in DNA repeat tract lengths. In NAR cancer, 4, zcac020. doi:10.1093/narcan/zcac020. https://pubmed.ncbi.nlm.nih.gov/35774233/
5. Yousefzadeh, Matthew J, Wood, Richard D. 2012. DNA polymerase POLQ and cellular defense against DNA damage. In DNA repair, 12, 1-9. doi:10.1016/j.dnarep.2012.10.004. https://pubmed.ncbi.nlm.nih.gov/23219161/
6. Wood, Richard D, Doublié, Sylvie. 2016. DNA polymerase θ (POLQ), double-strand break repair, and cancer. In DNA repair, 44, 22-32. doi:10.1016/j.dnarep.2016.05.003. https://pubmed.ncbi.nlm.nih.gov/27264557/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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