C57BL/6JCya-Polmem1/Cya
Common Name:
Polm-KO
Product ID:
S-KO-17644
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Polm-KO
Strain ID
KOCMP-54125-Polm-B6J-VB
Gene Name
Product ID
S-KO-17644
Gene Alias
B230309I03Rik; Tdt-N
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Polmem1/Cya mice (Catalog S-KO-17644) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020767
NCBI RefSeq
NM_017401
Target Region
Exon 2~11
Size of Effective Region
~9.4 kb
Detailed Document
Overview of Gene Research
Polm, also known as DNA polymerase mu, is a member of the DNA polymerase family. It is involved in DNA repair processes and plays a role in maintaining genomic stability. It has been associated with pathways such as the NF-κB signaling pathway, and is of biological importance in processes like tumorigenesis and host-virus interactions [1,2].
In ovarian cancer, the POLM G312R mutation promotes tumorigenesis. It increases the ribonucleotide insertion capability of POLM, leading to genomic instability. It also upregulates COL11A1, activates the NF-κB signaling pathway, and contributes to tumor progression and chemoresistance, identifying the POLM-COL11A1-NF-κB axis as a novel oncogenic pathway [1]. In porcine epidemic diarrhea virus (PEDV) infection, POLM expression increases, regulated by the transcription factor FOXA1. POLM targets and degrades PEDV structural proteins (N, S2, and M) via the autophagy pathway, inhibiting PEDV proliferation [2].
In conclusion, Polm is crucial for maintaining genomic stability and is involved in important biological processes related to cancer development and host-virus interactions. Studies on its role in ovarian cancer and PEDV infection using genetic models help understand its functions in these disease conditions, potentially providing new therapeutic targets for treatment [1,2].
References:
1. Xiao, Yue, Ni, Maowei, Zheng, Zhiguo, Xu, Hong, Hua, Yuejin. 2024. POLM variant G312R promotes ovarian tumorigenesis through genomic instability and COL11A1-NF-κB axis. In American journal of physiology. Cell physiology, 327, C168-C183. doi:10.1152/ajpcell.00025.2024. https://pubmed.ncbi.nlm.nih.gov/38826139/
2. Cao, Xinyu, Liu, Yingyu, Tong, Wu, Kong, Ning, Shan, Tongling. 2025. POLM inhibits porcine epidemic diarrhea virus replication by degrading multiple viral structural proteins. In Journal of virology, 99, e0227824. doi:10.1128/jvi.02278-24. https://pubmed.ncbi.nlm.nih.gov/39927776/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen