C57BL/6JCya-Prkag1em1/Cya
Common Name
Prkag1-KO
Product ID
S-KO-17653
Backgroud
C57BL/6JCya
Strain ID
KOCMP-19082-Prkag1-B6J-VB
When using this mouse strain in a publication, please cite “Prkag1-KO Mouse (Catalog S-KO-17653) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Prkag1-KO
Strain ID
KOCMP-19082-Prkag1-B6J-VB
Gene Name
Product ID
S-KO-17653
Gene Alias
Prkaac
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000168846
NCBI RefSeq
NM_016781.2
Target Region
Exon 3~12
Size of Effective Region
~3.3 kb
Overview of Gene Research
Prkag1, also known as protein kinase AMP-activated non-catalytic subunit gamma 1, is a crucial component of the AMP-activated protein kinase (AMPK) complex. AMPK plays a central role in cellular energy homeostasis, and Prkag1 is involved in sensing cellular nucleotide levels. It is associated with pathways such as autophagy, which is a key cellular stress response mechanism [1,2].
In the context of autophagy, DNA-dependent protein kinase (PRKDC) phosphorylates the nucleotide-sensing γ1 subunit Prkag1 at Ser192 and Thr284 in normal culture conditions. This phosphorylation primes the AMPK complex for lysosomal activation by STK11 in cancer cells, linking DNA damage response to autophagy and cellular metabolism [2].
Aging leads to a decline in Prkag1 expression in vertebrates, and transgenic fish with sustained AMPKγ1 show a more youthful feeding and fasting response, improved metabolic health and longevity. In humans, Prkag1 expression decline with age is associated with multimorbidity and frailty risk [3]. Also, a non-synonymous SNP (rs1126930) in PRKAG1 has been associated with developing chronic complex regional pain syndrome type 1 [4].
In conclusion, Prkag1 is essential for maintaining normal cellular metabolism and autophagy processes. Its role in aging-related metabolic decline and in the development of complex regional pain syndrome type 1 has been revealed through various studies. Understanding Prkag1's function can potentially provide insights into new strategies for promoting healthy aging and treating related diseases.
References：
1. Wu, Wenxian, Wang, Xiaojing, Sun, Yadong, Bergmann, Ann Kathrin, Stork, Björn. 2021. TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy. In Autophagy, 17, 3992-4009. doi:10.1080/15548627.2021.1899667. https://pubmed.ncbi.nlm.nih.gov/33779513/
2. Puustinen, Pietri, Keldsbo, Anne, Corcelle-Termeau, Elisabeth, Oakhill, Jon S, Jäättelä, Marja. 2020. DNA-dependent protein kinase regulates lysosomal AMP-dependent protein kinase activation and autophagy. In Autophagy, 16, 1871-1888. doi:10.1080/15548627.2019.1710430. https://pubmed.ncbi.nlm.nih.gov/31983282/
3. Ripa, Roberto, Ballhysa, Eugen, Steiner, Joachim D, Müller, Roman-Ulrich, Antebi, Adam. 2023. Refeeding-associated AMPKγ1 complex activity is a hallmark of health and longevity. In Nature aging, 3, 1544-1560. doi:10.1038/s43587-023-00521-y. https://pubmed.ncbi.nlm.nih.gov/37957359/
4. Shaikh, Samiha S, Goebel, Andreas, Lee, Michael C, Menon, David K, Woods, C Geoffrey. 2024. Evidence of a genetic background predisposing to complex regional pain syndrome type 1. In Journal of medical genetics, 61, 163-170. doi:10.1136/jmg-2023-109236. https://pubmed.ncbi.nlm.nih.gov/37816627/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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