C57BL/6JCya-Lctem1/Cya
Common Name:
Lct-KO
Product ID:
S-KO-17656
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Lct-KO
Strain ID
KOCMP-226413-Lct-B6J-VB
Gene Name
Product ID
S-KO-17656
Gene Alias
Gm100; LAC; LPH; Lphl
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lctem1/Cya mice (Catalog S-KO-17656) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000073490
NCBI RefSeq
NM_001081078
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Lct, or lactase, is an enzyme crucial for lactose digestion in the small intestine. It breaks down lactose into glucose and galactose, facilitating nutrient absorption. Its activity is associated with the LCT-13910C>T polymorphism 13910 bp upstream of the LCT-encoding gene [1,2,3].
Primary lactose intolerance (PLI), a decrease in lactase activity often seen from 1-5 years old, is related to single-nucleotide polymorphisms of the LCT gene. In children over 6 years old, the 13910CC and 22018GG LCT polymorphisms are significantly correlated with positive hydrogen breath test results, suggesting a potential for diagnosing lactose intolerance [1]. In the Italian and Turkish populations, the LCT-13910C>T polymorphism was not associated with an increased risk of colorectal cancer or polyps [2,3]. In the Armenian population, the lactase persistence allele LCT-13910T has a very low frequency (2.7%), supporting phenotypic data on lactose malabsorption there [4].
In conclusion, Lct is essential for lactose digestion. Genetic studies on Lct, especially those focusing on its polymorphisms, have provided insights into lactose intolerance and its potential relationship with colorectal cancer. These findings contribute to our understanding of digestive-related diseases and the genetic basis of lactose metabolism.
References:
1. Tomczonek-Moruś, J, Wojtasik, A, Zeman, K, Smolarz, B, Bąk-Romaniszyn, L. 2018. 13910C>T and 22018G>A LCT gene polymorphisms in diagnosing hypolactasia in children. In United European gastroenterology journal, 7, 210-216. doi:10.1177/2050640618814136. https://pubmed.ncbi.nlm.nih.gov/31080605/
2. Tarabra, Elena, Pazienza, Paola, Borghesio, Elisabetta, Comandone, Alessandro, Rosina, Floriano. 2010. LCT-13910C>T polymorphism-associated lactose malabsorption and risk for colorectal cancer in Italy. In Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 42, 741-3. doi:10.1016/j.dld.2010.02.013. https://pubmed.ncbi.nlm.nih.gov/20362522/
3. Gençdal, Genco, Salman, Esin, Özütemiz, Ömer, Akarca, Ulus S. 2017. Association of LCT-13910 C/T Polymorphism and Colorectal Cancer. In Annals of coloproctology, 33, 169-172. doi:10.3393/ac.2017.33.5.169. https://pubmed.ncbi.nlm.nih.gov/29159163/
4. Németh, Stefan, Kriegshäuser, Gernot, Hovhannesyan, Kristine, Oberkanins, Christian, Sarkisian, Tamara. 2022. Very low frequency of the lactase persistence allele LCT-13910T in the Armenian population. In Annals of human biology, 49, 260-262. doi:10.1080/03014460.2022.2126887. https://pubmed.ncbi.nlm.nih.gov/36129808/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen