C57BL/6JCya-Tmem176bem1/Cya
Common Name:
Tmem176b-KO
Product ID:
S-KO-17659
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmem176b-KO
Strain ID
KOCMP-65963-Tmem176b-B6J-VC
Gene Name
Product ID
S-KO-17659
Gene Alias
1810009M01Rik; Clast1; Lr8
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem176bem1/Cya mice (Catalog S-KO-17659) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000164733
NCBI RefSeq
NM_023056
Target Region
Exon 1~2
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Tmem176b, a member of the membrane spanning 4-domains (MS4) family, is an intracellular cation channel. It serves as a dual immunoregulator, being involved in modulating immune responses. It can both inhibit effector immune responses in certain settings and promote immunity by supporting antigen presentation. It also potentially regulates type 2 and 3 immunity, and is associated with pathways like inflammasome activation, DC biology modulation, and CD8+ T cell responses [1].
Disruption of Tmem176b in mice contributes to CD8+ T cell-mediated tumor growth inhibition by unleashing inflammasome activation, enhancing the antitumor activity of anti-CTLA-4 antibodies through caspase-1/IL-1β activation [2]. In triple-negative breast cancer, silencing Tmem176B in cell lines impaired cell proliferation, and in vivo studies showed attenuated tumor growth. The AKT/mTOR signaling pathway was repressed in Tmem176B-silenced cells [3]. In lung adenocarcinoma, Tmem176B promoted cellular functions like proliferation, invasion, and regulated EMT via the FGFR1/JNK/Vimentin/Snail signaling cascade [4]. In ovarian cancer, Tmem176B inhibited cancer progression by regulating EMT via the Wnt/β-catenin signaling pathway, with lower expression correlating with a worse prognosis [5].
In summary, Tmem176b plays a crucial role in immune regulation and is involved in multiple disease-related processes. Gene knockout or knockdown studies in mouse models and cell lines have revealed its significance in tumor immunity and cancer progression, providing potential therapeutic targets for cancer treatment. Its role in regulating key signaling pathways in different cancers and its dual function in immune responses highlight its importance in biomedical research [1-4, 7].
References:
1. Hill, Marcelo, Russo, Sofía, Olivera, Daniela, Galliussi, Germán, Segovia, Mercedes. 2022. The intracellular cation channel TMEM176B as a dual immunoregulator. In Frontiers in cell and developmental biology, 10, 1038429. doi:10.3389/fcell.2022.1038429. https://pubmed.ncbi.nlm.nih.gov/36340035/
2. Segovia, Mercedes, Russo, Sofia, Jeldres, Mathias, Rabinovich, Gabriel A, Hill, Marcelo. . Targeting TMEM176B Enhances Antitumor Immunity and Augments the Efficacy of Immune Checkpoint Blockers by Unleashing Inflammasome Activation. In Cancer cell, 35, 767-781.e6. doi:10.1016/j.ccell.2019.04.003. https://pubmed.ncbi.nlm.nih.gov/31085177/
3. Kang, Chifei, Rostoker, Ran, Ben-Shumel, Sarit, LeRoith, Derek, Gallagher, Emily Jane. 2021. TMEM176B Regulates AKT/mTOR Signaling and Tumor Growth in Triple-Negative Breast Cancer. In Cells, 10, . doi:10.3390/cells10123430. https://pubmed.ncbi.nlm.nih.gov/34943938/
4. Sun, Ping-Hui, Xia, Siyu, Yuan, Runzhu, Zhang, Bin, Wang, Guangsuo. 2024. TMEM176B Promotes EMT via FGFR/JNK Signalling in Development and Tumourigenesis of Lung Adenocarcinoma. In Cancers, 16, . doi:10.3390/cancers16132447. https://pubmed.ncbi.nlm.nih.gov/39001509/
5. Yan, Lili, Song, Zhaona, Yi, Lili, Yuan, Fengjiao, Jia, Dianlong. 2025. TMEM176B inhibits ovarian cancer progression by regulating EMT via the Wnt/β-catenin signaling pathway. In Journal of translational medicine, 23, 350. doi:10.1186/s12967-025-06362-0. https://pubmed.ncbi.nlm.nih.gov/40108613/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen