C57BL/6JCya-Hsd17b8em1/Cya
Common Name:
Hsd17b8-KO
Product ID:
S-KO-17670
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hsd17b8-KO
Strain ID
KOCMP-14979-Hsd17b8-B6J-VA
Gene Name
Product ID
S-KO-17670
Gene Alias
D17H6S112E; H-2Ke6; H2-Ke6; Ke-6; Ke6; Ring2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hsd17b8em1/Cya mice (Catalog S-KO-17670) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045467
NCBI RefSeq
NM_013543
Target Region
Exon 1~9
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
HSD17B8, also known as estradiol 17-beta-dehydrogenase 8, is an enzyme that converts estradiol to estrone through its NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity [1]. It is also involved in acyl thioester metabolism, participating in processes like mitochondrial fatty acid synthesis, peroxisomal β-oxidation of fatty acids, and mitochondrial oxidation of 3R-hydroxyacyl-groups [2,4].
In C. elegans, the loss of the PTEN homolog daf-18 triggers cell division during L1 arrest. Forward genetic screening identified F12E12.11, an ortholog of human HSD17B8, which impacts cell proliferation independently of PTEN's typical phosphatase activity. PTEN physically interacts with HSD17B8, and the reduction in estrone levels and accumulation of estradiol may arrest tumor cells in the G2/M phase of the cell cycle through MAPK/ERK [1]. In breast cancer, HSD17B8 is a protective gene; higher expression means a better prognosis [3].
In conclusion, HSD17B8 plays a significant role in steroid metabolism and cell proliferation regulation. Its interaction with PTEN provides insights into tumor suppression mechanisms. The protective role of HSD17B8 in breast cancer indicates its potential importance in cancer research, highlighting the value of model-based research in understanding its functions and implications in disease.
References:
1. Zhao, Wei, Huang, Ruiting, Ran, Dongyang, Qu, Zhi, Zheng, Shanqing. 2024. Inhibiting HSD17B8 suppresses the cell proliferation caused by PTEN failure. In Scientific reports, 14, 12280. doi:10.1038/s41598-024-63052-5. https://pubmed.ncbi.nlm.nih.gov/38811827/
2. Hiltunen, J Kalervo, Kastaniotis, Alexander J, Autio, Kaija J, Chen, Zhijun, Glumoff, Tuomo. 2018. 17B-hydroxysteroid dehydrogenases as acyl thioester metabolizing enzymes. In Molecular and cellular endocrinology, 489, 107-118. doi:10.1016/j.mce.2018.11.012. https://pubmed.ncbi.nlm.nih.gov/30508570/
3. Nie, Yunmeng, Huang, Fang, Lou, Lihua, Yan, Junbin. 2022. The Obesity-Related Metabolic Gene HSD17B8 Protects Against Breast Cancer: High RNA/Protein Expression Means a Better Prognosis. In Medical science monitor : international medical journal of experimental and clinical research, 28, e934424. doi:10.12659/MSM.934424. https://pubmed.ncbi.nlm.nih.gov/35184130/
4. Venkatesan, Rajaram, Sah-Teli, Shiv K, Awoniyi, Luqman O, Wierenga, Rik K, Chen, Zhijun. 2014. Insights into mitochondrial fatty acid synthesis from the structure of heterotetrameric 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase. In Nature communications, 5, 4805. doi:10.1038/ncomms5805. https://pubmed.ncbi.nlm.nih.gov/25203508/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen