C57BL/6JCya-Yod1em1/Cya
Common Name:
Yod1-KO
Product ID:
S-KO-17675
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Yod1-KO
Strain ID
KOCMP-226418-Yod1-B6J-VA
Gene Name
Product ID
S-KO-17675
Gene Alias
6330564D18Rik; 9930028C20Rik; Hshin7
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Yod1em1/Cya mice (Catalog S-KO-17675) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049813
NCBI RefSeq
NM_178691
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
YOD1, a 38 kDa protein belonging to the ovarian tumour protease (OTU) family, is a deubiquitinase. Ubiquitination is a reversible process, and YOD1, as a deubiquitinating enzyme (DUB), plays a role in controlling the ubiquitin-proteasome system by cleaving the bond between ubiquitin and substrate proteins. It is involved in multiple pathways such as cell-cycle regulation, p53, β-catenin, extracellular-regulated signal kinase (ERK), and YES-associated pathway (YAP) activities, which are crucial for various biological processes and disease developments [5].
In triple-negative breast cancer (TNBC), YOD1 is upregulated and functions as an oncogene. Knockdown of YOD1 in TNBC cells inhibits cell migration, proliferation, cell cycle, and drug resistance both in vitro and in vivo. Mechanistically, it binds with CDK1, de-polyubiquitylates CDK1, and upregulates its expression [1].
In colitis, YOD1-deficient mice are highly susceptible to dextran sulfate sodium (DSS)-induced colitis. YOD1 from hematopoietic cells inhibits DSS colitis by promoting NOD2-mediated physiological inflammation in macrophages, through stabilizing RIPK2 [2].
In MRSA sepsis-induced disseminated intravascular coagulation (DIC), YOD1 deficiency enhances NLRP3 inflammasome activation and coagulation. YOD1 interacts with NLRP3 to remove K33-linked ubiquitination of NLRP3, inhibiting its expression and inflammasome activation [3].
In head and neck squamous cell carcinoma (HNSCC), YOD1 is downregulated. Depletion of YOD1 promotes HNSCC growth, invasion, and epithelial-mesenchymal transition. YOD1 inhibits the ERK/β-catenin pathway by suppressing the ubiquitination and degradation of TRIM33 [4].
In conclusion, YOD1 has diverse functions in different biological processes and disease conditions. Its role in maintaining protein stability through deubiquitination affects various cellular activities. Gene knockout models, such as those in TNBC, colitis, MRSA-induced DIC, and HNSCC studies, have revealed its importance in these disease areas, providing potential therapeutic targets for treatment.
References：
1. Han, Zhitao, Jia, Qi, Zhang, Jing, Zha, Siluo, Zhang, Chunlei. 2023. Deubiquitylase YOD1 regulates CDK1 stability and drives triple-negative breast cancer tumorigenesis. In Journal of experimental & clinical cancer research : CR, 42, 228. doi:10.1186/s13046-023-02781-3. https://pubmed.ncbi.nlm.nih.gov/37667382/
2. Shen, Jiangyun, Lou, Liyan, Du, Xue, Ruan, Jing, Wang, Xu. 2024. YOD1 sustains NOD2-mediated protective signaling in colitis by stabilizing RIPK2. In EMBO reports, 25, 4827-4845. doi:10.1038/s44319-024-00276-6. https://pubmed.ncbi.nlm.nih.gov/39333628/
3. Liu, Chang, Fan, Caihong, Liu, Jia, Qi, Zhi, Shen, Yanna. 2024. YOD1 protects against MRSA sepsis-induced DIC through Lys33-linked deubiquitination of NLRP3. In Cell death & disease, 15, 360. doi:10.1038/s41419-024-06731-5. https://pubmed.ncbi.nlm.nih.gov/38789414/
4. Wu, Yue, Duan, Yuansheng, Han, Wei, Wang, Xudong, Jing, Chao. 2023. Deubiquitinase YOD1 suppresses tumor progression by stabilizing E3 ligase TRIM33 in head and neck squamous cell carcinoma. In Cell death & disease, 14, 517. doi:10.1038/s41419-023-06035-0. https://pubmed.ncbi.nlm.nih.gov/37573347/
5. Zhi-Xiong, Chong. 2025. Decoding YOD1: Insights into tumour regulation and translational opportunities. In Biochemical pharmacology, 236, 116889. doi:10.1016/j.bcp.2025.116889. https://pubmed.ncbi.nlm.nih.gov/40132762/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen