C57BL/6JCya-Trappc8em1/Cya
Common Name:
Trappc8-KO
Product ID:
S-KO-17687
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Trappc8-KO
Strain ID
KOCMP-75964-Trappc8-B6J-VB
Gene Name
Product ID
S-KO-17687
Gene Alias
5033403J15Rik; D030074E01Rik; Trs85; mKIAA1012
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trappc8em1/Cya mice (Catalog S-KO-17687) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000225661
NCBI RefSeq
NM_177038
Target Region
Exon 3~10
Size of Effective Region
~12.7 kb
Detailed Document
Overview of Gene Research
Trappc8, as a subunit of the transport protein particle (TRAPP) complex, is involved in multiple cellular processes. The TRAPP complex has different forms (TRAPPI, II, and III), and Trappc8 is specific to TRAPPIII. It functions in membrane traffic, autophagy, and ciliogenesis. In membrane traffic, TRAPPIII, with Trappc8 as a key subunit, acts as a guanine nucleotide exchange factor (GEF) for Rab GTPases, regulating vesicle transport. In autophagy, it is part of a complex required for proper autophagosome formation and the trafficking of Atg9A through recycling endosomes [3,4,5].
In ciliogenesis, Trappc8 has a distinct role. It interacts with the ciliopathy protein oral-facial-digital syndrome 1 (OFD1). Trappc8 is necessary for the association of OFD1 with pericentriolar material 1 (PCM1). Depletion of Trappc8 reduces the colocalized signals between OFD1 and PCM1, affecting ciliogenesis [1]. Also, Trappc8 has been identified as a host factor required for human papillomavirus (HPV) cell entry. Knockdown of Trappc8 in HeLa and HaCaT cells leads to reduced susceptibility to HPV infection, indicating its crucial role in the native HPV infection process [2].
In conclusion, Trappc8 is essential for various biological functions including membrane traffic, autophagy, and ciliogenesis. Its role in HPV cell entry also shows its significance in viral infection-related diseases. Studies on Trappc8, especially through loss-of-function experiments, help us understand its functions in these complex biological processes and disease-related mechanisms.
References:
1. Zhang, Caiyun, Li, Chunman, Siu, Gavin Ka Yu, Luo, Xiaomin, Yu, Sidney. 2020. Distinct Roles of TRAPPC8 and TRAPPC12 in Ciliogenesis via Their Interactions With OFD1. In Frontiers in cell and developmental biology, 8, 148. doi:10.3389/fcell.2020.00148. https://pubmed.ncbi.nlm.nih.gov/32258032/
2. Ishii, Yoshiyuki, Nakahara, Tomomi, Kataoka, Michiyo, Takeuchi, Takamasa, Kukimoto, Iwao. 2013. Identification of TRAPPC8 as a host factor required for human papillomavirus cell entry. In PloS one, 8, e80297. doi:10.1371/journal.pone.0080297. https://pubmed.ncbi.nlm.nih.gov/24244674/
3. Hoffman-Sommer, Marta, Piłka, Natalia, Anielska-Mazur, Anna, Dagdas, Yasin, Swiezewska, Ewa. . The TRAPPC8/TRS85 subunit of the Arabidopsis TRAPPIII tethering complex regulates endoplasmic reticulum function and autophagy. In Plant physiology, 197, . doi:10.1093/plphys/kiaf042. https://pubmed.ncbi.nlm.nih.gov/40084709/
4. Lamb, Christopher A, Nühlen, Stefanie, Judith, Delphine, Behrends, Christian, Tooze, Sharon A. 2015. TBC1D14 regulates autophagy via the TRAPP complex and ATG9 traffic. In The EMBO journal, 35, 281-301. doi:10.15252/embj.201592695. https://pubmed.ncbi.nlm.nih.gov/26711178/
5. Imai, Kenta, Hao, Feike, Fujita, Naonobu, Noda, Takeshi, Yoshimori, Tamotsu. 2016. Atg9A trafficking through the recycling endosomes is required for autophagosome formation. In Journal of cell science, 129, 3781-3791. doi:. https://pubmed.ncbi.nlm.nih.gov/27587839/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen