C57BL/6JCya-Cd79aem1/Cya
Common Name:
Cd79a-KO
Product ID:
S-KO-17734
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cd79a-KO
Strain ID
KOCMP-12518-Cd79a-B6J-VA
Gene Name
Product ID
S-KO-17734
Gene Alias
Ig-alpha; Iga; Igalpha; Ly-54; Ly54; mb-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cd79aem1/Cya mice (Catalog S-KO-17734) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003469
NCBI RefSeq
NM_007655
Target Region
Exon 2~3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Cd79a, also known as Igα, is a crucial signaling component of the preB cell receptor (preBCR) [1]. It is essential for B cell development as its successful expression marks the transition from pro-B to pre-B cells [4]. The BCR, composed of surface-bound Ig and a heterodimeric signaling unit of CD79A and CD79B, initiates important signals for B cell development, function, and tonic survival signaling. CD79A and CD79B are required for surface IgM expression in human B cells [3].
In a Cd79a-Cre;Tsc1ff mouse model (Cd79a-Tsc1 KO), Cre-mediated Tsc1 depletion driven by the Cd79a promoter activated mTORC1 exclusively along the distal nephron from embryonic day 16. This led to cyst formation in the distal nephron, suggesting that mTORC1 overactivation in developing distal tubules, mediated by Cd79a, disrupts morphogenesis and is involved in polycystic kidney disease-like cystogenesis [2]. In pediatric B-cell precursor acute lymphoblastic leukemia, downregulation of Cd79a in murine xenograft models hampered leukemia cell engraftment, especially in the central nervous system, indicating its role in CNS-infiltration and engraftment [1].
In conclusion, Cd79a is vital for B cell development and plays a role in maintaining B cell fitness through BCR-related functions. Mouse models, such as the Cd79a-Tsc1 KO and murine xenograft models, have revealed its significance in diseases like polycystic kidney disease and B-cell precursor acute lymphoblastic leukemia, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Lenk, Lennart, Carlet, Michela, Vogiatzi, Fotini, Alsadeq, Ameera, Schewe, Denis M. 2021. CD79a promotes CNS-infiltration and leukemia engraftment in pediatric B-cell precursor acute lymphoblastic leukemia. In Communications biology, 4, 73. doi:10.1038/s42003-020-01591-z. https://pubmed.ncbi.nlm.nih.gov/33452446/
2. Tran Nguyen Truc, Linh, Matsuda, Satoshi, Takenouchi, Akiko, Yoshizawa, Katsuhiko, Tsukaguchi, Hiroyasu. 2023. Mechanism of cystogenesis by Cd79a-driven, conditional mTOR activation in developing mouse nephrons. In Scientific reports, 13, 508. doi:10.1038/s41598-023-27766-2. https://pubmed.ncbi.nlm.nih.gov/36627370/
3. Huse, Kanutte, Bai, Baoyan, Hilden, Vera I, Wälchli, Sébastien, Myklebust, June H. . Mechanism of CD79A and CD79B Support for IgM+ B Cell Fitness through B Cell Receptor Surface Expression. In Journal of immunology (Baltimore, Md. : 1950), 209, 2042-2053. doi:10.4049/jimmunol.2200144. https://pubmed.ncbi.nlm.nih.gov/36426942/
4. Yu, Lang, Zhang, Yishi, Li, Wenhui, Zhao, Xiaodong, An, Yunfei. 2024. Fluoxetine Successfully Treats Intracranial Enterovirus E18 Infection in a Patient with CD79a Deficiency Arising from Segmental Uniparental Disomy of Chromosome 19. In Journal of clinical immunology, 44, 137. doi:10.1007/s10875-024-01740-7. https://pubmed.ncbi.nlm.nih.gov/38805163/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen