C57BL/6JCya-Fbxo25em1/Cya
Common Name
Fbxo25-KO
Product ID
S-KO-17750
Backgroud
C57BL/6JCya
Strain ID
KOCMP-66822-Fbxo25-B6J-VB
Status
When using this mouse strain in a publication, please cite “Fbxo25-KO Mouse (Catalog S-KO-17750) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Fbxo25-KO
Strain ID
KOCMP-66822-Fbxo25-B6J-VB
Gene Name
Product ID
S-KO-17750
Gene Alias
Fbx25, 9130015I06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000209913
NCBI RefSeq
NM_001347316
Target Region
Exon 4~5
Size of Effective Region
~2.9 kb
Overview of Gene Research
Fbxo25, an F-box protein, is a component of the SCF protein E3 ubiquitin ligase. It can specifically bind to substrates, regulating multiple biological processes. It is involved in pathways like MAPK signaling and has significance in cell differentiation, proliferation, and cancer-related processes [1,2]. Genetic models, such as gene knockout models, can be valuable for studying its functions.
In cutaneous squamous cell carcinoma (cSCC), stable silencing of Fbxo25 in SCC13 cells led to reduced tumor growth, accompanied by down-regulation of cyclin D1. This suggests Fbxo25 promotes cSCC growth and metastasis through cyclin D1 [1]. In NSCLC, FBXO25 was highly expressed in cancer samples, and its interference could inhibit cell proliferation, invasion, and migration in H1299 cells [4]. In the MAPK signaling pathway, FBXO25 functions as a negative regulator by decreasing MAPK/ERK activity through inhibition of ERK1/2 phosphorylation [2]. In cardiomyocyte development, Fbxo25-mediated SCF ubiquitination pathway regulates the protein levels and activities of Tbx5 and Nkx2-5, and silencing endogenous Fbxo25 suppresses mESC-derived cardiomyocyte differentiation [3].
In conclusion, Fbxo25 plays essential roles in multiple biological processes including cancer development and cardiomyocyte development. Model-based research, especially gene knockout models, has revealed its function in promoting tumor growth in cSCC and NSCLC, and its role in negatively regulating the MAPK signaling pathway and in cardiomyocyte development. This understanding contributes to the study of related diseases and may provide potential therapeutic targets.
References：
1. Kuzmanov, Aleksandar, Johansen, Pål, Hofbauer, Günther. 2020. FBXO25 Promotes Cutaneous Squamous Cell Carcinoma Growth and Metastasis through Cyclin D1. In The Journal of investigative dermatology, 140, 2496-2504. doi:10.1016/j.jid.2020.04.003. https://pubmed.ncbi.nlm.nih.gov/32335130/
2. Teixeira, Felipe R, Manfiolli, Adriana O, Vieira, Nichelle A, Schechtman, Deborah, Gomes, Marcelo D. 2017. FBXO25 regulates MAPK signaling pathway through inhibition of ERK1/2 phosphorylation. In Archives of biochemistry and biophysics, 621, 38-45. doi:10.1016/j.abb.2017.04.003. https://pubmed.ncbi.nlm.nih.gov/28389297/
3. Jeong, Hoe-Su, Jung, Eun-Shil, Sim, Ye-Ji, Kim, Chang-Hoon, Kim, Kye-Seong. 2015. Fbxo25 controls Tbx5 and Nkx2-5 transcriptional activity to regulate cardiomyocyte development. In Biochimica et biophysica acta, 1849, 709-21. doi:10.1016/j.bbagrm.2015.02.002. https://pubmed.ncbi.nlm.nih.gov/25725482/
4. Jiang, Gui-Yang, Zhang, Xiu-Peng, Wang, Liang, Wang, En-Hua, Zhang, Yong. 2016. FBXO25 promotes cell proliferation, invasion, and migration of NSCLC. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37, 14311-14319. doi:. https://pubmed.ncbi.nlm.nih.gov/27596142/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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