C57BL/6JCya-Ppp1r14bem1/Cya
Common Name:
Ppp1r14b-KO
Product ID:
S-KO-17758
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ppp1r14b-KO
Strain ID
KOCMP-18938-Ppp1r14b-B6J-VB
Gene Name
Product ID
S-KO-17758
Gene Alias
AOM172; PHI-1; PLCB3N; Png
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp1r14bem1/Cya mice (Catalog S-KO-17758) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070850
NCBI RefSeq
NM_008889
Target Region
Exon 2~4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ppp1r14b, protein phosphatase 1 regulatory subunit 14B, is a poorly defined member of the protein phosphatase 1 regulatory subunits. It can inhibit protein phosphatase 1 (PP1) and various PP1 holoenzymes, which are crucial proteins in regulating cell growth, the cell cycle, and apoptosis. PPP1R14B is involved in multiple cellular pathways, and its abnormal expression is associated with various diseases, especially cancers [1,2].
In triple-negative breast cancer, PPP1R14B was upregulated, promoting cell proliferation, colony formation, migration, invasion, and paclitaxel resistance both in vitro and in vivo. Mechanistically, it maintained phosphorylation and stability of STMN1, a microtubule-destabilizing phosphoprotein [1].
In uterine corpus endometrial carcinoma, higher PPP1R14B expression was associated with poorer overall survival, disease-specific survival, and progression-free interval [2].
In prostate cancer, it was a potential diagnostic and prognostic biomarker, with its overexpression related to tumor immune inhibition, proliferation, and migration [3].
In hepatocellular carcinoma, PPP1R14B was highly expressed, promoting tumorigenesis by maintaining the stability and phosphorylation of RPS6KA1 and positively regulating the AKT/NF-κB pathway [4].
In cervical and endometrial cancers, PPP1R14B knockdown repressed cell proliferation and facilitated cell death by inhibiting the Akt signaling pathway [5].
In kidney renal clear cell carcinoma, upregulated PPP1R14B was an independent risk factor for cancer progression, and its knockdown reduced cell proliferation and migration in vitro. It was also associated with tumor-infiltrating immune cells [6].
In conclusion, PPP1R14B plays a significant role in cancer progression, often promoting tumor-related processes such as cell proliferation, migration, and drug resistance. Its involvement in multiple cancer types makes it a potential therapeutic target. Research on PPP1R14B using gene knockout or conditional knockout models, as inferred from the findings on its functional impacts, helps in understanding its role in cancer-related biological processes, thus contributing to the development of new cancer treatment strategies.
References：
1. Liao, Li, Zhang, Yin-Ling, Deng, Ling, Shao, Zhi-Min, Li, Da-Qiang. . Protein Phosphatase 1 Subunit PPP1R14B Stabilizes STMN1 to Promote Progression and Paclitaxel Resistance in Triple-Negative Breast Cancer. In Cancer research, 83, 471-484. doi:10.1158/0008-5472.CAN-22-2709. https://pubmed.ncbi.nlm.nih.gov/36484700/
2. He, Kang, Wang, Taiwei, Huang, Xuemiao, Jiang, Junjie, Zhao, Lijing. 2023. PPP1R14B is a diagnostic prognostic marker in patients with uterine corpus endometrial carcinoma. In Journal of cellular and molecular medicine, 27, 846-863. doi:10.1111/jcmm.17697. https://pubmed.ncbi.nlm.nih.gov/36824011/
3. Bao, Yizhong, Ni, Yixiu, Zhang, Aokang, Chen, Jun. 2024. PPP1R14B as a potential biomarker for the identification of diagnosis and prognosis affecting tumor immunity, proliferation and migration in prostate cancer. In Journal of Cancer, 15, 6545-6564. doi:10.7150/jca.101100. https://pubmed.ncbi.nlm.nih.gov/39668827/
4. Zhou, Nana, Guo, Chaoqin, Du, Jingyang, Zheng, Xiaoliang, Tu, Linglan. 2024. PPP1R14B-mediated phosphorylation enhances protein stability of RPS6KA1 to promote hepatocellular carcinoma tumorigenesis. In Biochimica et biophysica acta. Molecular cell research, 1871, 119840. doi:10.1016/j.bbamcr.2024.119840. https://pubmed.ncbi.nlm.nih.gov/39216602/
5. Xiang, Nan, Chen, Tao, Zhao, Xiaoli, Zhao, Min. 2022. In vitro assessment of roles of PPP1R14B in cervical and endometrial cancer. In Tissue & cell, 77, 101845. doi:10.1016/j.tice.2022.101845. https://pubmed.ncbi.nlm.nih.gov/35679681/
6. Cheng, Lang, Mi, Junhao, Zhang, Jiange, Huang, Houbao, Mo, Zengnan. 2023. Upregulated PPP1R14B is connected to cancer progression and immune infiltration in kidney renal clear cell carcinoma. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 26, 119-135. doi:10.1007/s12094-023-03228-z. https://pubmed.ncbi.nlm.nih.gov/37261660/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen