C57BL/6JCya-Dhrs7cem1/Cya
Common Name:
Dhrs7c-KO
Product ID:
S-KO-17766
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dhrs7c-KO
Strain ID
KOCMP-68460-Dhrs7c-B6J-VB
Gene Name
Product ID
S-KO-17766
Gene Alias
1110001P11Rik; SRP-35
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dhrs7cem1/Cya mice (Catalog S-KO-17766) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000168612
NCBI RefSeq
NM_001013013
Target Region
Exon 3~7
Size of Effective Region
~6.2 kb
Detailed Document
Overview of Gene Research
DHRS7c, also known as SRP-35, is a short-chain dehydrogenase/reductase (SDR) localized to the endo/sarcoplasmic reticulum. It is highly expressed in heart and skeletal muscle, and is involved in maintaining intracellular Ca²⁺ homeostasis and glucose metabolism [1,2,3]. It may be associated with pathways related to muscle function, heart failure, and certain metabolic processes. Genetic models, such as gene knockout mouse models, have been crucial in studying its functions.
In C2C12 cells, functional loss of DHRS7C (using knockout cells) leads to intracellular Ca²⁺ overload and myotube enlargement via calpain activation, indicating its role in maintaining Ca²⁺ balance in muscle cells [2]. In skeletal muscle, over-expression of DHRS7C (SRP-35) activates mTORC2, enhances glucose metabolism and muscle performance, showing its importance in muscle metabolism [3]. In heart-related studies, its expression is down-regulated by adrenergic stimulation and in heart failure models, suggesting its possible role in heart failure development [1].
In conclusion, DHRS7c is essential for maintaining intracellular Ca²⁺ homeostasis in muscle cells and enhancing glucose metabolism in skeletal muscle. Its down-regulation in heart failure models indicates its potential significance in heart failure research. The use of gene knockout models has been instrumental in revealing these functions and associations, which contribute to our understanding of muscle-related and heart-related disease mechanisms.
References:
1. Lu, Bo, Tigchelaar, Wardit, Ruifrok, Willem P T, de Boer, Rudolf A, Silljé, Herman H W. 2011. DHRS7c, a novel cardiomyocyte-expressed gene that is down-regulated by adrenergic stimulation and in heart failure. In European journal of heart failure, 14, 5-13. doi:10.1093/eurjhf/hfr152. https://pubmed.ncbi.nlm.nih.gov/22143674/
2. Arai, Shinobu, Ikeda, Masataka, Ide, Tomomi, Sunagawa, Kenji, Tsutsui, Hiroyuki. 2016. Functional loss of DHRS7C induces intracellular Ca2+ overload and myotube enlargement in C2C12 cells via calpain activation. In American journal of physiology. Cell physiology, 312, C29-C39. doi:10.1152/ajpcell.00090.2016. https://pubmed.ncbi.nlm.nih.gov/27806939/
3. Ruiz, Alexis, Dror, Erez, Handschin, Christoph, Treves, Susan, Zorzato, Francesco. 2018. Over-expression of a retinol dehydrogenase (SRP35/DHRS7C) in skeletal muscle activates mTORC2, enhances glucose metabolism and muscle performance. In Scientific reports, 8, 636. doi:10.1038/s41598-017-18844-3. https://pubmed.ncbi.nlm.nih.gov/29330505/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen