C57BL/6JCya-Bmal1em1/Cya
Common Name:
Bmal1-KO
Product ID:
S-KO-17769
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Bmal1-KO
Strain ID
KOCMP-11865-Bmal1-B6J-VB
Gene Name
Product ID
S-KO-17769
Gene Alias
Arnt3; Arntl; BMAL1b; MOP3; bHLHe5; bmal1b'
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bmal1em1/Cya mice (Catalog S-KO-17769) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047321
NCBI RefSeq
NM_007489
Target Region
Exon 6~8
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Bmal1, also known as Brain and muscle arnt-like protein 1, is a crucial transcription factor that is a core component of the circadian oscillation machinery. It forms a heterodimer with Clock and binds to the E-box element in the promoters of genes like Period circadian protein (Per) and Cryptochrome (Cry), regulating the rhythmic expression of circadian clock control genes. Bmal1 is involved in multiple biological processes, with its dysregulation associated with various diseases [4].
In diabetic cardiomyopathy, Bmal1 downregulation in cardiomyocyte-specific knockout mice (CKB) and type 2 diabetes (T2D) mice accelerates cardiac hypertrophy and diastolic dysfunction, while overexpression ameliorates these pathological changes. Bmal1 seems to act through the Bcl2/IP3R-mediated mitochondrial Ca2+ overload pathway [1].
In sepsis-induced acute lung injury, BMAL1 deficiency in macrophages exacerbates systemic inflammation and lung injury by upregulating CXCL2 expression, which increases neutrophil recruitment and NETs formation via binding to CXCR2 [2].
In sepsis-induced acute kidney injury (AKI), decreased BMAL1 expression is observed, and its overexpression alleviates renal tubular injury by reducing ferroptosis levels in renal tubular epithelial cells through inhibiting YAP expression and the Hippo pathway [3].
In conclusion, Bmal1 plays a vital role in maintaining normal physiological functions, especially in regulating circadian rhythms. Its dysregulation is associated with multiple diseases such as diabetic cardiomyopathy, sepsis-related organ injuries. Gene knockout mouse models, like CKB for Bmal1, have been instrumental in revealing its role in these disease conditions, providing potential therapeutic targets for treatment.
References:
1. Zhang, Nannan, Yu, Hao, Liu, Tianzi, Hou, Xiaofeng, Zou, Jiangang. 2023. Bmal1 downregulation leads to diabetic cardiomyopathy by promoting Bcl2/IP3R-mediated mitochondrial Ca2+ overload. In Redox biology, 64, 102788. doi:10.1016/j.redox.2023.102788. https://pubmed.ncbi.nlm.nih.gov/37356134/
2. Zeng, Ting, Liang, Long, Deng, Wenjun, Liu, Jing, Yang, Minghua. 2024. BMAL1 plays a crucial role in immune homeostasis during sepsis-induced acute lung injury. In Biochemical pharmacology, 226, 116379. doi:10.1016/j.bcp.2024.116379. https://pubmed.ncbi.nlm.nih.gov/38908531/
3. Yang, Songyuan, Ye, Zehua, Chen, Wu, Li, Wei, Cheng, Fan. 2024. BMAL1 alleviates sepsis-induced AKI by inhibiting ferroptosis. In International immunopharmacology, 142, 113159. doi:10.1016/j.intimp.2024.113159. https://pubmed.ncbi.nlm.nih.gov/39303541/
4. Fan, Xu-Li, Song, Ying, Qin, Dong-Xu, Lin, Pei-Yao. 2021. Regulatory Effects of Clock and Bmal1 on Circadian Rhythmic TLR Expression. In International reviews of immunology, 42, 101-112. doi:10.1080/08830185.2021.1931170. https://pubmed.ncbi.nlm.nih.gov/34544330/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen