C57BL/6JCya-Crygsem1/Cya
Common Name:
Crygs-KO
Product ID:
S-KO-17775
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Crygs-KO
Strain ID
KOCMP-12970-Crygs-B6J-VB
Gene Name
Product ID
S-KO-17775
Gene Alias
Opj; rncat
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crygsem1/Cya mice (Catalog S-KO-17775) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040592
NCBI RefSeq
NM_009967.2
Target Region
Exon 2~3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Crygs, the gene encoding gamma-S crystallin, is a crucial gene as gamma-S crystallin is one of the major structural proteins in the eye lens, playing a vital role in maintaining lens transparency [2,3,4]. It is also expressed in non-lens tissues like the retina and cornea in some species, suggesting additional non-crystallin roles, perhaps related to stress response [4].
In mice, a mutation in Crygs causes the Opj cataract. The Opj mutation replaces Phe-9 with serine, leading to a temperature-sensitive phenotype where the mutant protein folds normally at low temperature but has reduced solubility and loss of secondary structure near physiological temperatures. This is similar to processes in human senile cataracts. In Opj/Opj mice, cortical fiber cell morphology and the loss of maturing fiber cell nuclei are severely disrupted, indicating a loss of gamma-S crystallin function related to maintaining cytoarchitecture [2]. In a Chinese family, a novel heterozygous missense mutation c.199T > A, p.(Tyr67Asn) in CRYGS was identified, which was predicted to decrease local hydrophobicity, affect the three-dimensional structure of γS-crystallin, and cause partial mutant protein translocation from the cytoplasm to the cell membrane, resulting in congenital cataract [1]. A G18V mutation in human gammaS-crystallin associated with autosomal dominant cortical progressive cataract makes the protein more sensitive to thermal and chemical stress, which is consistent with the progressive cataract formation in family members carrying this mutation [3].
In conclusion, Crygs is essential for maintaining the normal structure and function of the eye lens. Studies on mouse models with Crygs mutations, like the Opj cataract model, have revealed its role in preventing cataract formation. Understanding the function of Crygs through these models provides insights into the molecular mechanisms of cataractogenesis, which may contribute to the development of strategies for cataract prevention and treatment.
References:
1. Zhang, Tianxiao, Yan, Lulu, Leng, Yunji, Zhang, Jinsong, Cao, Lihua. 2018. A novel missense mutation of CRYGS underlies congenital cataract in a Chinese family. In Gene, 675, 9-14. doi:10.1016/j.gene.2018.06.100. https://pubmed.ncbi.nlm.nih.gov/29964096/
2. Sinha, D, Wyatt, M K, Sarra, R, Lyon, M, Wistow, G. 2000. A temperature-sensitive mutation of Crygs in the murine Opj cataract. In The Journal of biological chemistry, 276, 9308-15. doi:. https://pubmed.ncbi.nlm.nih.gov/11121426/
3. Ma, Zhiwei, Piszczek, Grzegorz, Wingfield, Paul T, Sergeev, Yuri V, Hejtmancik, J Fielding. . The G18V CRYGS mutation associated with human cataracts increases gammaS-crystallin sensitivity to thermal and chemical stress. In Biochemistry, 48, 7334-41. doi:10.1021/bi900467a. https://pubmed.ncbi.nlm.nih.gov/19558189/
4. Sinha, D, Esumi, N, Jaworski, C, Pierce, E, Wistow, G. 1998. Cloning and mapping the mouse Crygs gene and non-lens expression of [gamma]S-crystallin. In Molecular vision, 4, 8. doi:. https://pubmed.ncbi.nlm.nih.gov/9565648/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen