C57BL/6JCya-Ptrh1em1/Cya
Common Name:
Ptrh1-KO
Product ID:
S-KO-17788
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ptrh1-KO
Strain ID
KOCMP-329384-Ptrh1-B6J-VA
Gene Name
Product ID
S-KO-17788
Gene Alias
2210013M04Rik; PTH
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptrh1em1/Cya mice (Catalog S-KO-17788) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066352
NCBI RefSeq
NM_178595
Target Region
Exon 2~5
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Ptrh1, or peptidyl-tRNA hydrolase 1 homolog, is an RNA binding protein (RBP) [1]. It is involved in the ribosome-associated quality control (RQC) pathway, where it can cleave non-ubiquitinated NC-tRNA in non-ubiquitinated 60S ribosome-nascent chain complexes (60S RNCs) and 80S RNCs formed on non-stop mRNAs, suggesting a pathway for the release of non-ubiquitinated nascent chains (NCs) [2].
In pancreatic cancer, high glucose downregulates Ptrh1 through RAS signaling following EGFR stimulation. This downregulation enhances the stability of the PD-L1 mRNA, leading to increased PD-L1 expression. Overexpression of Ptrh1 in pancreatic cells suppresses PD-L1 expression and improves the proportion and cytotoxic function of CD8+ T cells in the pancreatic tumor microenvironment (TME) of diabetic mice, indicating its importance in anti-tumor immunity in the pancreatic TME [1].
In colorectal cancer, Ptrh1 is one of the eight prognosis-related RBPs identified. It was selected, along with three other RBPs, to construct a prognostic risk score model that accurately predicted the prognosis of CRC patients [3]. In another study, Ptrh1 was included in a five-gene prognostic signature for CRC, suggesting its role in the pathogenesis and prognosis of CRC [4].
In conclusion, Ptrh1 is a significant RBP involved in the RQC pathway and has implications in multiple disease conditions. Studies, especially those using mouse models in pancreatic and colorectal cancer, have revealed its crucial role in tumor-related immune regulation and prognosis, providing insights into potential therapeutic strategies for these diseases.
References:
1. Gao, Chenggang, Chen, Jiaoshun, Bai, Jianwei, Shen, Qiang, Yin, Tao. 2023. High glucose-upregulated PD-L1 expression through RAS signaling-driven downregulation of PTRH1 leads to suppression of T cell cytotoxic function in tumor environment. In Journal of translational medicine, 21, 461. doi:10.1186/s12967-023-04302-4. https://pubmed.ncbi.nlm.nih.gov/37434177/
2. Kuroha, Kazushige, Zinoviev, Alexandra, Hellen, Christopher U T, Pestova, Tatyana V. 2018. Release of Ubiquitinated and Non-ubiquitinated Nascent Chains from Stalled Mammalian Ribosomal Complexes by ANKZF1 and Ptrh1. In Molecular cell, 72, 286-302.e8. doi:10.1016/j.molcel.2018.08.022. https://pubmed.ncbi.nlm.nih.gov/30244831/
3. Zhang, Zhen, Wang, Ling, Wang, Quan, Wang, Shan, Shen, Zhanlong. 2020. Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer. In Frontiers in genetics, 11, 580149. doi:10.3389/fgene.2020.580149. https://pubmed.ncbi.nlm.nih.gov/33193701/
4. Liang, Qiankun, Du, Xiaojuan, Mao, Lanfang, Wang, Guopan. . Molecular characterization of colorectal cancer: A five-gene prognostic signature based on RNA-binding proteins. In Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association, 27, 223-233. doi:10.4103/sjg.sjg_530_20. https://pubmed.ncbi.nlm.nih.gov/34169901/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen