C57BL/6JCya-Zfpm1em1/Cya
Common Name:
Zfpm1-KO
Product ID:
S-KO-17796
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfpm1-KO
Strain ID
KOCMP-22761-Zfpm1-B6J-VA
Gene Name
Product ID
S-KO-17796
Gene Alias
FOG; Fog1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfpm1em1/Cya mice (Catalog S-KO-17796) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054052
NCBI RefSeq
NM_009569
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Zfpm1, also known as Friend of GATA1 (FOG-1), is an essential transcriptional co-factor. It is involved in multiple biological processes, playing a crucial role in cell differentiation, development, and in maintaining cellular homeostasis. It associates with GATA transcription factors and is part of the regulatory machinery for gene expression in various cell lineages [4,5].
In zebrafish, zfpm1 knockout leads to compromised cardiac function with deformed trabecular meshwork. The loss of Zfpm1 activity results in over-activation of Neuregulin-ErbB signalling and abnormally elevated cardiomyocyte proliferation during cardiac trabeculae growth and modelling stages, indicating its role in coordinating trabeculae patterning and growth [1].
In mice, conditional knockout of Zfpm1 affects the development of dorsal raphe serotonergic neuron subtypes. In perinatal and adult mutants, there are changes in the number of serotonergic neurons in different subpopulations of the dorsal raphe nucleus, reduced serotonin concentration in rostral brain areas, increased anxiety-like behavior, and in female mutants, elevated contextual fear memory [3].
In breast cancer research, next-generation sequencing of encapsulated papillary carcinoma (EPC) reveals that 21.9% of EPCs have somatic ZFPM1 mutations, often concurrent with PI3K-AKT-mTOR pathway mutations, suggesting a role in this specific cancer type [2].
In conclusion, Zfpm1 is vital for normal development, especially in cardiac trabeculation and the development of serotonergic neuron subtypes. The study of Zfpm1 knockout and conditional knockout models in zebrafish and mice has provided insights into its role in cardiac function and mood-related behaviors. In addition, the discovery of ZFPM1 mutations in EPC indicates its potential significance in breast cancer. These model-based studies contribute to understanding the biological functions of Zfpm1 and its implications in disease.
References:
1. Yang, Yuxi, Li, Beibei, Zhang, Xue, Zhao, Qinshun, Lou, Xin. 2019. The zinc finger protein Zfpm1 modulates ventricular trabeculation through Neuregulin-ErbB signalling. In Developmental biology, 446, 142-150. doi:10.1016/j.ydbio.2019.01.001. https://pubmed.ncbi.nlm.nih.gov/30611731/
2. Liu, Xuguang, Huang, Xin, Bai, Yan, Wu, Huanwen, Liang, Zhiyong. 2021. Next-generation sequencing revealed recurrent ZFPM1 mutations in encapsulated papillary carcinoma of the breast. In NPJ precision oncology, 5, 42. doi:10.1038/s41698-021-00180-5. https://pubmed.ncbi.nlm.nih.gov/34007008/
3. Tikker, Laura, Casarotto, Plinio, Singh, Parul, Castrén, Eero, Partanen, Juha. 2020. Inactivation of the GATA Cofactor ZFPM1 Results in Abnormal Development of Dorsal Raphe Serotonergic Neuron Subtypes and Increased Anxiety-Like Behavior. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 40, 8669-8682. doi:10.1523/JNEUROSCI.2252-19.2020. https://pubmed.ncbi.nlm.nih.gov/33046550/
4. Wilkinson-White, Lorna, Gamsjaeger, Roland, Dastmalchi, Siavoush, Mackay, Joel P, Matthews, Jacqueline M. 2011. Structural basis of simultaneous recruitment of the transcriptional regulators LMO2 and FOG1/ZFPM1 by the transcription factor GATA1. In Proceedings of the National Academy of Sciences of the United States of America, 108, 14443-8. doi:10.1073/pnas.1105898108. https://pubmed.ncbi.nlm.nih.gov/21844373/
5. Roussis, Ioannis-Marios, Pearton, David J, Niazi, Umar, Ragoussis, Jiannis, Strouboulis, John. 2025. A novel role for Friend of GATA1 (FOG-1) in regulating cholesterol transport in murine erythropoiesis. In PLoS genetics, 21, e1011617. doi:10.1371/journal.pgen.1011617. https://pubmed.ncbi.nlm.nih.gov/40048486/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen