C57BL/6NCya-Got2em1/Cya
Common Name:
Got2-KO
Product ID:
S-KO-17800
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Got2-KO
Strain ID
KOCMP-14719-Got2-B6N-VA
Gene Name
Product ID
S-KO-17800
Gene Alias
FABP-pm; Got-2; Kyat4; mAspAT
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Got2em1/Cya mice (Catalog S-KO-17800) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034097
NCBI RefSeq
NM_010325
Target Region
Exon 2~3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
GOT2, also known as Glutamic-oxaloacetic transaminase 2, is a highly tissue-specific gene in the liver. It is a key component of the malate-aspartate shuttle, which is crucial for maintaining intracellular NAD(H) redox balance and amino acid metabolism [3,4]. This shuttle mechanism enables cells to transfer reducing equivalents from the cytosol to the mitochondria, and GOT2 is also involved in mutant KRAS-mediated rewiring of glutamine metabolism in pancreatic ductal adenocarcinoma [2].
In hepatocellular carcinoma (HCC), knockdown of GOT2 in HCC cells promoted proliferation, migration, invasion, and in mouse models of HCC, loss of GOT2 promoted tumor growth as well as hematogenous and intrahepatic metastasis. Mechanistically, it enhanced glutaminolysis, nucleotide synthesis, and glutathione synthesis by reprogramming glutamine metabolism, activating the PI3K/AKT/mTOR pathway [1].
In pancreatic cancer, the loss of GOT2 in vitro disturbed redox homeostasis and halted proliferation of pancreatic ductal adenocarcinoma (PDA) cells. However, in xenograft PDA or autochthonous mouse models, loss of GOT2 had no effect on tumor growth as the pancreatic tumor microenvironment, specifically cancer-associated fibroblasts releasing pyruvate, could rescue the proliferation of GOT2-knockdown cells [2].
In conclusion, GOT2 plays a significant role in maintaining cellular redox balance and amino acid metabolism through its involvement in the malate-aspartate shuttle. Gene knockout studies in mouse models have revealed its complex role in cancer development, showing differential effects in vitro and in vivo depending on the tumor microenvironment. These findings provide insights into potential therapeutic and diagnostic applications for HCC and pancreatic cancer [1,2].
References:
1. Li, Yunzheng, Li, Binghua, Xu, Yanchao, Sun, Beicheng, Yu, Decai. . GOT2 Silencing Promotes Reprogramming of Glutamine Metabolism and Sensitizes Hepatocellular Carcinoma to Glutaminase Inhibitors. In Cancer research, 82, 3223-3235. doi:10.1158/0008-5472.CAN-22-0042. https://pubmed.ncbi.nlm.nih.gov/35895805/
2. Kerk, Samuel A, Lin, Lin, Myers, Amy L, Shah, Yatrik M, Lyssiotis, Costas A. 2022. Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context. In eLife, 11, . doi:10.7554/eLife.73245. https://pubmed.ncbi.nlm.nih.gov/35815941/
3. van Karnebeek, Clara D M, Ramos, Rúben J, Wen, Xiao-Yan, Zaki, Maha S, Wevers, Ron A. 2019. Bi-allelic GOT2 Mutations Cause a Treatable Malate-Aspartate Shuttle-Related Encephalopathy. In American journal of human genetics, 105, 534-548. doi:10.1016/j.ajhg.2019.07.015. https://pubmed.ncbi.nlm.nih.gov/31422819/
4. Bu, Jiarui, Miao, Zeyu, Yang, Qing. 2024. GOT2: New therapeutic target in pancreatic cancer. In Genes & diseases, 12, 101370. doi:10.1016/j.gendis.2024.101370. https://pubmed.ncbi.nlm.nih.gov/40247913/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen