C57BL/6JCya-Gcdhem1/Cya
Common Name:
Gcdh-KO
Product ID:
S-KO-17802
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gcdh-KO
Strain ID
KOCMP-270076-Gcdh-B6J-VC
Gene Name
Product ID
S-KO-17802
Gene Alias
9030411L18; GCD
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gcdhem1/Cya mice (Catalog S-KO-17802) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003907
NCBI RefSeq
NM_008097
Target Region
Exon 3~7
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Gcdh, or glutaryl-CoA dehydrogenase, is a mitochondrial protein crucial for lysine metabolism. It functions in converting glutaryl-CoA to crotonyl-CoA, thereby controlling protein glutarylation [1,2,3]. This process is integral to lysine catabolism and impacts multiple biological processes. Genetic models, such as knockout mouse models, have been instrumental in studying Gcdh's functions.
In melanoma cells, Gcdh knockdown induced cell death, which was blocked by inhibiting the upstream lysine catabolism enzyme DHTKD1. Mechanistically, Gcdh knockdown led to NRF2 glutarylation, increasing its stability and DNA binding activity, and upregulating genes like ATF4, ATF3, DDIT3, and CHAC1 [1].
In glioblastoma stem cells, upregulation of Gcdh along with SLC7A2 and downregulation of ECHS1 reprogrammed lysine catabolism, leading to intracellular crotonyl-CoA accumulation and histone H4 lysine crotonylation [2].
In hepatocellular carcinoma, Gcdh depletion promoted tumor growth and metastasis, while overexpression reversed these processes. Gcdh-induced crotonylation of PPP and glycolysis-related proteins limited the Warburg effect and induced senescence, shaping an anti-tumor immune microenvironment [3].
In conclusion, Gcdh is essential for lysine metabolism and protein glutarylation. Model-based research, especially Gcdh knockout mouse models, has revealed its role in diseases such as melanoma, glioblastoma, and hepatocellular carcinoma. These findings provide potential therapeutic targets for treating these malignancies.
References:
1. Verma, Sachin, Crawford, David, Khateb, Ali, Jackson, Michael, Ronai, Ze'ev A. 2022. NRF2 mediates melanoma addiction to GCDH by modulating apoptotic signalling. In Nature cell biology, 24, 1422-1432. doi:10.1038/s41556-022-00985-x. https://pubmed.ncbi.nlm.nih.gov/36050469/
2. Yuan, Huairui, Wu, Xujia, Wu, Qiulian, Snyder, Nathaniel W, Rich, Jeremy N. 2023. Lysine catabolism reprograms tumour immunity through histone crotonylation. In Nature, 617, 818-826. doi:10.1038/s41586-023-06061-0. https://pubmed.ncbi.nlm.nih.gov/37198486/
3. Lao, Yuanxiang, Cui, Xiaohan, Xu, Zhu, Qin, Xihu, Sun, Beicheng. 2024. Glutaryl-CoA dehydrogenase suppresses tumor progression and shapes an anti-tumor microenvironment in hepatocellular carcinoma. In Journal of hepatology, 81, 847-861. doi:10.1016/j.jhep.2024.05.034. https://pubmed.ncbi.nlm.nih.gov/38825017/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen