C57BL/6JCya-Sytl2em1/Cya
Common Name:
Sytl2-KO
Product ID:
S-KO-17803
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Sytl2-KO
Strain ID
KOCMP-83671-Sytl2-B6J-VB
Gene Name
Product ID
S-KO-17803
Gene Alias
Slp2; mKIAA1597
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sytl2em1/Cya mice (Catalog S-KO-17803) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000190731
NCBI RefSeq
NM_001289583
Target Region
Exon 12~13
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Sytl2, or synaptotagmin-like 2, has been implicated in multiple biological processes and disease-related pathways. Although its exact essential function is still being elucidated, it has connections to cell migration, invasion, and immune-related processes. In cell migration, it may be involved in cytoskeletal-related mechanisms, and in the immune context, it could potentially play a role in immune cell regulation and function [1,2,7].
In prostate cancer, SYTL2 promotes metastasis by enhancing FSCN1-mediated pseudopodia formation and invasion. It binds to and inhibits the proteasome degradation pathway of FSCN1, increasing its stability and leading to enhanced cell migration, invasion, and lymph node metastasis both in vitro and in vivo [1].
In thoracic aortic aneurysm, SYTL2, along with COL1A1, may be diagnostic marker genes. Their upregulation might be involved in inflammatory infiltration of the vessel wall and poor extracellular matrix remodeling, promoting the disease progression [2].
In spinal osteosarcoma, SYTL2 is part of the miR-654-3p/SYTL2 axis targeted by lncRNA BSN-AS2, which promotes cancer progression [3].
In ovarian cancer, overexpression of SYTL2 promotes metastatic potential, including increased migration and invasiveness [4].
In alveolar soft-part sarcoma, Sytl2 is involved in the intracellular trafficking of bioactive signals during angiogenesis [5,6]. Additionally, SYTL2 has been identified as a potential diagnostic biomarker for sleep-deprivation related myocardial infarction, and is strongly associated with resting NK cells and NK cell signaling pathways [7].
In conclusion, Sytl2 plays crucial roles in cancer metastasis, disease diagnosis, and potentially in immune-related diseases. Studies on Sytl2, especially through in vivo models in cancer research, have provided valuable insights into its functions in promoting cancer cell migration and invasion, as well as its potential as a diagnostic biomarker in non-cancerous diseases. This knowledge can contribute to the development of new therapeutic strategies and diagnostic methods for related diseases.
References：
1. Li, Zean, Tao, Yiran, Gao, Ze, Chen, Xu, Huang, Hai. 2023. SYTL2 promotes metastasis of prostate cancer cells by enhancing FSCN1-mediated pseudopodia formation and invasion. In Journal of translational medicine, 21, 303. doi:10.1186/s12967-023-04146-y. https://pubmed.ncbi.nlm.nih.gov/37147713/
2. Xie, Xinsheng, Yuan, Ye, Huang, Yulong, Fu, Weiguo, Wang, Lixin. 2023. Effects of COL1A1 and SYTL2 on inflammatory cell infiltration and poor extracellular matrix remodeling of the vascular wall in thoracic aortic aneurysm. In Chinese medical journal, 137, 1105-1114. doi:10.1097/CM9.0000000000002808. https://pubmed.ncbi.nlm.nih.gov/37640670/
3. Zhou, Xianwei, Li, Jitian, Teng, Junyan, Liu, Linyun, Zhang, Wenming. 2020. Long noncoding RNA BSN-AS2 induced by E2F1 promotes spinal osteosarcoma progression by targeting miR-654-3p/SYTL2 axis. In Cancer cell international, 20, 133. doi:10.1186/s12935-020-01205-y. https://pubmed.ncbi.nlm.nih.gov/32351327/
4. Sung, Hye Youn, Han, Jihye, Ju, Woong, Ahn, Jung-Hyuck. 2016. Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer. In Oncology reports, 36, 535-41. doi:10.3892/or.2016.4835. https://pubmed.ncbi.nlm.nih.gov/27220283/
5. Chuaychob, Surachada, Lyu, Ruyin, Tanaka, Miwa, Nakamura, Takuro, Yokokawa, Ryuji. 2024. Mimicking angiogenic microenvironment of alveolar soft-part sarcoma in a microfluidic coculture vasculature chip. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2312472121. doi:10.1073/pnas.2312472121. https://pubmed.ncbi.nlm.nih.gov/38502703/
6. Tanaka, Miwa, Chuaychob, Surachada, Homme, Mizuki, Yokokawa, Ryuji, Nakamura, Takuro. 2023. ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma. In Nature communications, 14, 1957. doi:10.1038/s41467-023-37049-z. https://pubmed.ncbi.nlm.nih.gov/37029109/
7. Chen, Xiang, Li, Qian, Zhang, Zhong, Yang, Minjing, Wang, E. 2022. Identification of Potential Diagnostic Biomarkers From Circulating Cells During the Course of Sleep Deprivation-Related Myocardial Infarction Based on Bioinformatics Analyses. In Frontiers in cardiovascular medicine, 9, 843426. doi:10.3389/fcvm.2022.843426. https://pubmed.ncbi.nlm.nih.gov/35369343/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen