C57BL/6JCya-Nup93em1/Cya
Common Name:
Nup93-KO
Product ID:
S-KO-17814
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nup93-KO
Strain ID
KOCMP-71805-Nup93-B6J-VB
Gene Name
Product ID
S-KO-17814
Gene Alias
2410008G02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nup93em1/Cya mice (Catalog S-KO-17814) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000212824
NCBI RefSeq
NM_172410
Target Region
Exon 8~9
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Nup93, a crucial structural protein of the nuclear pore complex (NPC), mediates nucleocytoplasmic shuttling of transcription factors. NPCs play a vital role in controlling the transportation of RNAs, proteins, and other macromolecules between the nucleus and cytoplasm, thereby influencing various biological processes [6,9].
Endothelial Nup93 protein levels decline in aged mice and in vitro models of endothelial aging. Loss of Nup93 impairs NPC transport, leading to nuclear accumulation of Yap and downstream inflammation, suggesting its importance in preventing endothelial cell senescence and vascular aging [1]. In breast cancer, Nup93 depletion reduces cell migration/proliferation, impairs invasion of triple-negative breast cancer cells, and leads to significant defects in tumor establishment/propagation in vivo [2]. In cardiomyocytes, down-regulation of Nup93 aggravates hypoxia-induced injury and cell death through abnormal regulation of gene transcription, mainly affecting oxidative phosphorylation and ribosome subunits-related genes [6]. Mutations in NUP93 cause steroid-resistant nephrotic syndrome in children, with most patients progressing to end-stage kidney disease [3,4,7]. In cervical cancer, knockdown of Nup93 inhibits cell proliferation, migration, and invasion capacity, and prevents tumor formation in mice [8]. In antiviral innate immune responses, Nup93-deficient cells show decreased expression of antiviral genes and impaired IRF3 nuclear translocation, as Nup93 enhances TBK1 activity [5].
In summary, Nup93 is essential for maintaining normal cellular functions. Studies using gene-knockout or other loss-of-function models have revealed its significant roles in various disease conditions such as endothelial cell senescence, breast cancer, cardiomyocyte hypoxia-induced death, steroid-resistant nephrotic syndrome, cervical cancer, and antiviral innate immunity. Understanding Nup93's functions provides potential therapeutic targets for these diseases.
References:
1. Nguyen, Tung D, Rao, Mihir K, Dhyani, Shaiva P, Michalkiewicz, Julka, Lee, Monica Y. 2023. Nucleoporin93 (Nup93) Limits Yap Activity to Prevent Endothelial Cell Senescence. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.11.10.566598. https://pubmed.ncbi.nlm.nih.gov/38014013/
2. Bersini, Simone, Lytle, Nikki K, Schulte, Roberta, Wahl, Geoffrey M, Hetzer, Martin W. 2020. Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodeling. In Life science alliance, 3, . doi:10.26508/lsa.201900623. https://pubmed.ncbi.nlm.nih.gov/31959624/
3. Han, Yanxinli, Sha, Hongyu, Yang, Yuan, Zhang, Yu, Zhou, Jianhua. 2024. Mutations in the NUP93, NUP107 and NUP160 genes cause steroid-resistant nephrotic syndrome in Chinese children. In Italian journal of pediatrics, 50, 81. doi:10.1186/s13052-024-01656-3. https://pubmed.ncbi.nlm.nih.gov/38650033/
4. Wasilewska, Anna, Rybi-Szuminska, Agnieszka, Dubiela, Pawel. 2023. Steroid-Resistant Nephrotic Syndrome Caused by NUP93 Pathogenic Variants. In Journal of clinical medicine, 12, . doi:10.3390/jcm12185810. https://pubmed.ncbi.nlm.nih.gov/37762751/
5. Monwan, Warunthorn, Kawasaki, Takumi, Hasan, Md Zobaer, Ori, Daisuke, Kawai, Taro. 2019. Identification of nucleoporin 93 (Nup93) that mediates antiviral innate immune responses. In Biochemical and biophysical research communications, 521, 1077-1082. doi:10.1016/j.bbrc.2019.11.035. https://pubmed.ncbi.nlm.nih.gov/31733835/
6. Pan, Lei, Song, Xiao-Wei, Song, Jin-Chao, Zhao, Xian-Xian, Ge, Jun-Bo. 2023. Downregulation of NUP93 aggravates hypoxia-induced death of cardiomyocytes in vitro through abnormal regulation of gene transcription. In Acta pharmacologica Sinica, 44, 969-983. doi:10.1038/s41401-022-01036-9. https://pubmed.ncbi.nlm.nih.gov/36807413/
7. Bierzynska, Agnieszka, Bull, Katherine, Miellet, Sara, Hartley, Paul S, Saleem, Moin A. 2022. Exploring the relevance of NUP93 variants in steroid-resistant nephrotic syndrome using next generation sequencing and a fly kidney model. In Pediatric nephrology (Berlin, Germany), 37, 2643-2656. doi:10.1007/s00467-022-05440-5. https://pubmed.ncbi.nlm.nih.gov/35211795/
8. Ouyang, Xiaolan, Hao, Xiaoming, Liu, Shuaibin, Hu, Jianguo, Hu, Lina. . Expression of Nup93 is associated with the proliferation, migration and invasion capacity of cervical cancer cells. In Acta biochimica et biophysica Sinica, 51, 1276-1285. doi:10.1093/abbs/gmz131. https://pubmed.ncbi.nlm.nih.gov/31774908/
9. Vollmer, Benjamin, Antonin, Wolfram. . The diverse roles of the Nup93/Nic96 complex proteins - structural scaffolds of the nuclear pore complex with additional cellular functions. In Biological chemistry, 395, 515-28. doi:10.1515/hsz-2013-0285. https://pubmed.ncbi.nlm.nih.gov/24572986/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen