C57BL/6JCya-Mestem1/Cya
Common Name
Mest-KO
Product ID
S-KO-17824
Backgroud
C57BL/6JCya
Strain ID
KOCMP-17294-Mest-B6J-VB
When using this mouse strain in a publication, please cite “Mest-KO Mouse (Catalog S-KO-17824) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mest-KO
Strain ID
KOCMP-17294-Mest-B6J-VB
Gene Name
Product ID
S-KO-17824
Gene Alias
Peg1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000124665
NCBI RefSeq
NM_001252292
Target Region
Exon 4~8
Size of Effective Region
~3.0 kb
Overview of Gene Research
Mest, short for Mesoderm-specific transcript, has been implicated in various biological processes and disease conditions. It has been associated with pathways like IL-6/JAK/STAT3/Twist-1 in breast cancer and STAT3/Twist-1-mediated EMT in bladder cancer, playing a role in tumorigenicity and metastasis [2,3]. Its loss of imprinting has also been linked to certain cancers through promoter switching [2].
High MEST expression in esophageal squamous cell carcinoma (ESCC) was associated with poor patient survival and promoted cancer invasion and metastasis. Mechanistically, it activates SRCIN1/RASAL1-ERK-snail signaling by interacting with PURA, and miR-449a, which is a direct regulator of MEST, has its promoter hypermethylated leading to MEST upregulation. Systemically delivered miR-449a mimic could suppress tumor metastasis without overt toxicity. Additionally, a compound G699-0288 was found to target the MEST-PURA interaction and significantly inhibit cancer metastasis [1]. In breast and bladder cancers, MEST depletion experiments showed that it is required for promoting cell proliferation, migration, invasion, and EMT [2,3].
In conclusion, Mest is involved in important biological processes related to tumorigenesis and metastasis, especially in cancers such as ESCC, breast, and bladder cancers. Functional studies through gene depletion in these cancer models have revealed its oncogenic roles, highlighting its potential as a therapeutic target for cancer treatment.
References：
1. Xu, Wen Wen, Liao, Long, Dai, Wei, He, Qing-Yu, Li, Bin. 2023. Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasis. In EBioMedicine, 92, 104587. doi:10.1016/j.ebiom.2023.104587. https://pubmed.ncbi.nlm.nih.gov/37149929/
2. Kim, Min Soo, Lee, Hyun Sook, Kim, Yun Jae, Kang, Sung Gyun, Jin, Wook. 2019. MEST induces Twist-1-mediated EMT through STAT3 activation in breast cancers. In Cell death and differentiation, 26, 2594-2606. doi:10.1038/s41418-019-0322-9. https://pubmed.ncbi.nlm.nih.gov/30903102/
3. Zhao, Cheng, Hu, Xiheng, Tong, Shiyu, Wang, Long, Li, Yangle. . MEST promotes bladder cancer cell proliferation, migration and invasion via STAT3/Twist-1-mediated EMT. In Translational cancer research, 9, 6178-6188. doi:10.21037/tcr-20-1006. https://pubmed.ncbi.nlm.nih.gov/35117228/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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