C57BL/6JCya-Pole3em1/Cya
Common Name
Pole3-KO
Product ID
S-KO-17870
Backgroud
C57BL/6JCya
Strain ID
KOCMP-59001-Pole3-B6J-VA
When using this mouse strain in a publication, please cite “Pole3-KO Mouse (Catalog S-KO-17870) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Pole3-KO
Strain ID
KOCMP-59001-Pole3-B6J-VA
Gene Name
Product ID
S-KO-17870
Gene Alias
1810034K18Rik, YBL1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 4
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000030091
NCBI RefSeq
NM_021498
Target Region
Exon 1~4
Size of Effective Region
~2.4 kb
Overview of Gene Research
POLE3, also known as DPB4/YBL1/CHRAC17, is one of the subunits of DNA polymerase ε. It contains a histone-like domain and is involved in multiple biological processes. POLE3 plays a role in chromatin-related functions, DNA repair, and replication-associated processes, which are crucial for maintaining genomic stability and cell-type-specific development [6,3,4].
Knockdown and knockout experiments have revealed that POLE3 is a transcriptional repressor of unintegrated HIV-1 DNA, maintaining it in a repressive chromatin state, which is important for efficient virus integration, escape from innate immune sensing, and optimal viral replication [1]. Loss of POLE3-POLE4 strongly sensitizes cancer cells to PARP inhibitors in a Polε level-independent manner, triggering replicative gap accumulation in a PRIMPOL-dependent manner [2]. POLE3 interacts with CHRAC1 to promote DNA repair, and its transcription is sensitive to the deposition of H2BK120ub1 and H3K79me2 [3]. Cells deficient in POLE3 show compromised H3K9me3 asymmetry and increased LINE expression [5]. Mice lacking Pole3 survive up to late embryonic stages, and hypomorphic and neomorphic alleles reveal a stage-specific function of Pole3 during T and B cell development [7].
In conclusion, POLE3 is essential for maintaining chromatin integrity, DNA repair, and replication-related processes. Its role in transcriptional repression of unintegrated HIV-1 DNA and sensitivity to PARP inhibitors in cancer cells, as well as its function in lymphocyte development, indicates its significance in both viral-host interactions and cancer-related research. Studies using gene knockout models have been instrumental in uncovering these functions, providing valuable insights into potential therapeutic targets for treating viral infections and cancers.
References：
1. Thenin-Houssier, Suzie, Machida, Shinichi, Jahan, Cyprien, Cuvier, Olivier, Benkirane, Monsef. 2023. POLE3 is a repressor of unintegrated HIV-1 DNA required for efficient virus integration and escape from innate immune sensing. In Science advances, 9, eadh3642. doi:10.1126/sciadv.adh3642. https://pubmed.ncbi.nlm.nih.gov/37922361/
2. Hill, Bethany Rebekah, Ozgencil, Meryem, Buckley-Benbow, Lauryn, Agnarelli, Alessandro, Bellelli, Roberto. 2024. Loss of POLE3-POLE4 unleashes replicative gap accumulation upon treatment with PARP inhibitors. In Cell reports, 43, 114205. doi:10.1016/j.celrep.2024.114205. https://pubmed.ncbi.nlm.nih.gov/38753485/
3. Mao, Xiaobing, Wu, Jian, Zhang, Qin, Liu, Cong, Han, Junhong. 2023. Requirement of WDR70 for POLE3-mediated DNA double-strand breaks repair. In Science advances, 9, eadh2358. doi:10.1126/sciadv.adh2358. https://pubmed.ncbi.nlm.nih.gov/37682991/
4. Bellelli, Roberto, Belan, Ondrej, Pye, Valerie E, Almouzni, Genevieve, Boulton, Simon J. 2018. POLE3-POLE4 Is a Histone H3-H4 Chaperone that Maintains Chromatin Integrity during DNA Replication. In Molecular cell, 72, 112-126.e5. doi:10.1016/j.molcel.2018.08.043. https://pubmed.ncbi.nlm.nih.gov/30217558/
5. Li, Zhiming, Duan, Shoufu, Hua, Xu, Goff, Stephen P, Zhang, Zhiguo. 2023. Asymmetric distribution of parental H3K9me3 in S phase silences L1 elements. In Nature, 623, 643-651. doi:10.1038/s41586-023-06711-3. https://pubmed.ncbi.nlm.nih.gov/37938774/
6. Bolognese, Fabrizio, Forni, Claudia, Caretti, Giuseppina, Minuzzo, Mario, Mantovani, Roberto. 2006. The Pole3 bidirectional unit is regulated by MYC and E2Fs. In Gene, 366, 109-16. doi:. https://pubmed.ncbi.nlm.nih.gov/16403426/
7. Siamishi, Iliana, Iwanami, Norimasa, Clapes, Thomas, O'Meara, Connor P, Boehm, Thomas. . Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles. In Cell reports, 31, 107756. doi:10.1016/j.celrep.2020.107756. https://pubmed.ncbi.nlm.nih.gov/32553171/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.