C57BL/6JCya-Ldhdem1/Cya
Common Name:
Ldhd-KO
Product ID:
S-KO-17880
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ldhd-KO
Strain ID
KOCMP-52815-Ldhd-B6J-VB
Gene Name
Product ID
S-KO-17880
Gene Alias
4733401P21Rik; D8Bwg1320e; DLD
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ldhdem1/Cya mice (Catalog S-KO-17880) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070004
NCBI RefSeq
NM_027570
Target Region
Exon 1~8
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Ldhd, lactate dehydrogenase D, is an enzyme that catalyzes the oxidation of D-lactate to pyruvate [3]. It plays a crucial role in the metabolism of D-2-hydroxyacids, and is associated with D-lactic acidosis [3]. Genetic models, such as gene knockout in mice, can be used to study its function.
In esophageal squamous cell carcinoma (ESCC), the CDK7-YAP-Ldhd axis promotes D-lactate elimination and ferroptosis defense, which supports cancer stem cell-like properties [1]. High Ldhd status is connected with poor prognosis in ESCC patients [1].
In hepatocellular carcinoma, Ldhd plays an important role in the prognosis of patients. Knockdown of Ldhd in HepG2 and Huh7 cells promoted cell proliferation, migration, and invasion, while overexpression showed the opposite effects [2].
In patients with neurological manifestations and mitochondrial complex IV deficiency, mutations in Ldhd lead to human D-lactate dehydrogenase deficiency and elevated serum D-lactate levels [4].
In conclusion, Ldhd is essential for D-lactate metabolism. Studies using knockout models have revealed its significant roles in various diseases, including ESCC and hepatocellular carcinoma, highlighting its potential as a therapeutic target for these diseases.
References：
1. Lv, Mengzhu, Gong, Ying, Liu, Xuesong, Zhang, Weimin, Zhan, Qimin. 2023. CDK7-YAP-LDHD axis promotes D-lactate elimination and ferroptosis defense to support cancer stem cell-like properties. In Signal transduction and targeted therapy, 8, 302. doi:10.1038/s41392-023-01555-9. https://pubmed.ncbi.nlm.nih.gov/37582812/
2. Wang, Shengnan, Wu, Xingwei, Wu, Xiaoming, Chen, Qianyi, Qi, Zhilin. 2024. Systematic analysis of the role of LDHs subtype in pan-cancer demonstrates the importance of LDHD in the prognosis of hepatocellular carcinoma patients. In BMC cancer, 24, 156. doi:10.1186/s12885-024-11920-8. https://pubmed.ncbi.nlm.nih.gov/38291366/
3. Jin, Shan, Chen, Xingchen, Yang, Jun, Ding, Jianping. 2023. Lactate dehydrogenase D is a general dehydrogenase for D-2-hydroxyacids and is associated with D-lactic acidosis. In Nature communications, 14, 6638. doi:10.1038/s41467-023-42456-3. https://pubmed.ncbi.nlm.nih.gov/37863926/
4. Kwong, Anna Ka-Yee, Wong, Sheila Suet-Na, Rodenburg, Richard J T, Chan, Godfrey Chi Fung, Fung, Cheuk-Wing. 2021. Human d-lactate dehydrogenase deficiency by LDHD mutation in a patient with neurological manifestations and mitochondrial complex IV deficiency. In JIMD reports, 60, 15-22. doi:10.1002/jmd2.12220. https://pubmed.ncbi.nlm.nih.gov/34258137/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen