C57BL/6JCya-Gabrb1em1/Cya
Common Name
Gabrb1-KO
Product ID
S-KO-17899
Backgroud
C57BL/6JCya
Strain ID
KOCMP-14400-Gabrb1-B6J-VA
When using this mouse strain in a publication, please cite “Gabrb1-KO Mouse (Catalog S-KO-17899) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gabrb1-KO
Strain ID
KOCMP-14400-Gabrb1-B6J-VA
Gene Name
Product ID
S-KO-17899
Gene Alias
B230208N19Rik, Gabrb-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031122
NCBI RefSeq
NM_008069
Target Region
Exon 4
Size of Effective Region
~2.9 kb
Overview of Gene Research
Gabrb1, encoding the beta1 subunit of the gamma-aminobutyric acid A receptor (GABA A receptor), is responsible for mediating inhibitory neurotransmission in the thalamus [3]. The GABAergic pathway, in which Gabrb1 is involved, plays a crucial role in maintaining the balance between neuronal excitation and inhibition in the brain [2].
In Gabrb1-/-mouse models, autistic-like behaviors were observed. RNA-seq and single-cell RNA sequencing revealed up-regulation of Glul/Slc38a3 in astrocytes, Grin1/Grin2b in neurons, glutamate, and the Glu/GABA ratio in the hippocampus. Increased NMDAR-currents and reduced GABAAR-currents in CA1 neurons were detected. NMDAR antagonist memantine or Glul inhibitor methionine sulfoximine could rescue the abnormal behaviors, suggesting that upregulation of the glutamatergic synapse pathway may lead to autistic-like behaviors [1].
In conclusion, Gabrb1 is essential for mediating inhibitory neurotransmission in the thalamus. The Gabrb1-/-mouse model has provided valuable insights into the role of Gabrb1 in the development of autistic-like behaviors, highlighting the importance of the balance between glutamatergic and GABAergic systems in normal brain function.
References：
1. Wang, Jing, Gao, Yue, Xiao, Liuyan, Zhong, Mei, Yang, Xinping. 2023. Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1-/- mice. In iScience, 26, 107476. doi:10.1016/j.isci.2023.107476. https://pubmed.ncbi.nlm.nih.gov/37599823/
2. Feng, Yan, Wei, Zi-Han, Liu, Chao, Zhang, Chu-Chu, Deng, Yan-Chun. 2022. Genetic variations in GABA metabolism and epilepsy. In Seizure, 101, 22-29. doi:10.1016/j.seizure.2022.07.007. https://pubmed.ncbi.nlm.nih.gov/35850019/
3. Zhu, Bi, Chen, Chuansheng, Xue, Gui, Dong, Qi, Lin, Chongde. 2014. The GABRB1 gene is associated with thalamus volume and modulates the association between thalamus volume and intelligence. In NeuroImage, 102 Pt 2, 756-63. doi:10.1016/j.neuroimage.2014.08.048. https://pubmed.ncbi.nlm.nih.gov/25192656/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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