C57BL/6JCya-Hif1anem1/Cya
Common Name:
Hif1an-KO
Product ID:
S-KO-17905
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hif1an-KO
Strain ID
KOCMP-319594-Hif1an-B6J-VA
Gene Name
Product ID
S-KO-17905
Gene Alias
2310046M24Rik; A830014H24Rik; FIH; FIH1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hif1anem1/Cya mice (Catalog S-KO-17905) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040455
NCBI RefSeq
NM_176958
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Hif1an, also known as hypoxia-inducible factor 1-alpha subunit suppressor, plays a crucial role in regulating hypoxia-inducible factor 1-alpha (HIF-1α) stability and transcriptional action. It is part of the oxygen-sensing pathway, with the PHD family of oxygen-dependent prolyl hydroxylases and FIH1-mediated asparagine hydroxylation also involved in tuning HIF transcriptional activity [2]. Hif1an is important in various biological processes, and its inappropriate expression is associated with cancer development and immune control [1].
In breast cancer, low Hif1an expression accompanied by less immune infiltration is associated with poor prognosis. High Hif1an expression is linked to good overall survival, and its expression is closely related to chemokines and immune cell infiltration [1].
In keloid formation, circSLC8A1 regulates the miR-181a-5p/HIF1AN axis to restrain the biological functions of human keloid fibroblasts [3].
In cardiomyocytes, pinoresinol diglucoside ameliorates hypoxia/reperfusion-induced injury by regulating miR-142-3p and HIF1AN [4].
In choriocarcinoma, METTL3 promotes miR-21-5p maturation to accelerate choriocarcinoma progression via the HIF1AN-induced inactivation of the HIF1A/VEGF pathway [5].
In hypertrophic scar formation, Rynchopeterine inhibits the formation of hypertrophic scars by regulating the miR-21/HIF1AN axis [6].
In skin flap transplantation, M2 macrophage-derived exosomes induce angiogenesis and increase skin flap survival through HIF1AN/HIF-1α/VEGFA control [7].
In osteoblast differentiation, miR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells [8].
In conclusion, Hif1an is essential for regulating HIF-1α, which impacts multiple biological processes. Its dysregulation is involved in various diseases such as cancer, keloid, and hypertrophic scar formation, as well as in processes like angiogenesis and osteoblast differentiation. Studies on Hif1an contribute to understanding the underlying mechanisms of these diseases and biological processes, potentially providing new strategies for treatment and prevention.
References：
1. Tang, Shasha, Liu, Dongyang, Fang, Yuan, Wang, Hui, Cai, Fengfeng. 2023. Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer. In Frontiers in oncology, 13, 1080910. doi:10.3389/fonc.2023.1080910. https://pubmed.ncbi.nlm.nih.gov/36816977/
2. Kaelin, William G, Ratcliffe, Peter J. . Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. In Molecular cell, 30, 393-402. doi:10.1016/j.molcel.2008.04.009. https://pubmed.ncbi.nlm.nih.gov/18498744/
3. Yuan, Xiaoye, Chen, Baiye, Wang, Xueming. 2022. CircSLC8A1 targets miR-181a-5p/HIF1AN pathway to inhibit the growth, migration and extracellular matrix deposition of human keloid fibroblasts. In Burns : journal of the International Society for Burn Injuries, 49, 622-632. doi:10.1016/j.burns.2022.04.009. https://pubmed.ncbi.nlm.nih.gov/35610079/
4. Wei, Yuan, Xiao, Liang, Yingying, Liu, Haichen, Wang. 2022. Pinoresinol diglucoside ameliorates H/R-induced injury of cardiomyocytes by regulating miR-142-3p and HIF1AN. In Journal of biochemical and molecular toxicology, 36, e23175. doi:10.1002/jbt.23175. https://pubmed.ncbi.nlm.nih.gov/35962614/
5. Ye, Kefan, Li, Lingchuan, Wu, Bao, Wang, Dongjie. 2022. METTL3 m6A-dependently promotes miR-21-5p maturation to accelerate choriocarcinoma progression via the HIF1AN-induced inactivation of the HIF1A/VEGF pathway. In Genes & genomics, 44, 1311-1322. doi:10.1007/s13258-022-01309-x. https://pubmed.ncbi.nlm.nih.gov/36074324/
6. Zhao, Wenbin, Ye, Jianzhou, Yang, Xuesong, Zhang, Qiongyu, Li, Jiaqi. 2024. Rynchopeterine inhibits the formation of hypertrophic scars by regulating the miR-21/HIF1AN axis. In Experimental cell research, 440, 114114. doi:10.1016/j.yexcr.2024.114114. https://pubmed.ncbi.nlm.nih.gov/38823472/
7. Luo, Gaojie, Zhou, Zekun, Cao, Zheming, Tang, Juyu, Qing, Liming. 2023. M2 macrophage-derived exosomes induce angiogenesis and increase skin flap survival through HIF1AN/HIF-1α/VEGFA control. In Archives of biochemistry and biophysics, 751, 109822. doi:10.1016/j.abb.2023.109822. https://pubmed.ncbi.nlm.nih.gov/38030054/
8. Yin, Nuo, Zhu, Longzhang, Ding, Liang, Xue, Feng, Xiao, Haijun. 2019. MiR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells. In Cellular & molecular biology letters, 24, 51. doi:10.1186/s11658-019-0177-6. https://pubmed.ncbi.nlm.nih.gov/31410089/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen