C57BL/6JCya-Idnkem1/Cya
Common Name:
Idnk-KO
Product ID:
S-KO-17909
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Idnk-KO
Strain ID
KOCMP-75731-Idnk-B6J-VB
Gene Name
Product ID
S-KO-17909
Gene Alias
5133401N09Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Idnkem1/Cya mice (Catalog S-KO-17909) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000051490
NCBI RefSeq
NM_198004.3
Target Region
Exon 2~3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Idnk, as gluconokinase, is involved in the phosphorylation of gluconate to generate 6-phosphogluconate, which is further metabolized via the Entner-Doudoroff pathway in Escherichia coli. In this bacterium, it is part of the GntII system related to L-idonic acid catabolism [2,3].
In hepatocellular carcinoma (HCC) cells, knockdown of IDNK significantly inhibited cell proliferation and increased apoptosis. A total of 1196 genes were differentially expressed in IDNK-knockdown BEL-7404 cells, and the pathway of tRNA charging was significantly inhibited. This indicates IDNK may be a candidate therapeutic target for HCC [1].
In conclusion, Idnk has a role in metabolism-related pathways in bacteria. In the context of HCC, its function in promoting cell proliferation and inhibiting apoptosis was revealed through knockdown experiments, suggesting its potential significance in cancer treatment research.
References：
1. Wu, Xiao-Min, Jin, Cheng, Gu, Yuan-Long, Zhang, Shuo, Zhang, Zhen. 2020. Gluconokinase IDNK Promotes Cell Proliferation and Inhibits Apoptosis in Hepatocellular Carcinoma. In OncoTargets and therapy, 13, 1767-1776. doi:10.2147/OTT.S234055. https://pubmed.ncbi.nlm.nih.gov/32161472/
2. Bausch, Christoph, Ramsey, Matthew, Conway, Tyrrell. . Transcriptional organization and regulation of the L-idonic acid pathway (GntII system) in Escherichia coli. In Journal of bacteriology, 186, 1388-97. doi:. https://pubmed.ncbi.nlm.nih.gov/14973046/
3. Bausch, C, Peekhaus, N, Utz, C, Lowary, T, Conway, T. . Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli. In Journal of bacteriology, 180, 3704-10. doi:. https://pubmed.ncbi.nlm.nih.gov/9658018/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen