C57BL/6JCya-Incenpem1/Cya
Common Name:
Incenp-KO
Product ID:
S-KO-17911
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Incenp-KO
Strain ID
KOCMP-16319-Incenp-B6J-VA
Gene Name
Product ID
S-KO-17911
Gene Alias
2700067E22Rik; C130081E20
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Incenpem1/Cya mice (Catalog S-KO-17911) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025562
NCBI RefSeq
NM_016692
Target Region
Exon 4
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Incenp, short for Inner Centromere Protein, is crucial for correct chromosome segregation during mitosis. It is a key component of the chromosomal passenger complex (CPC), which has diverse functions in cell division. Incenp connects the kinase Aurora B to the inner centromere, allowing the kinase to access its kinetochore targets. Additionally, an ATM-Chk2-Incenp pathway has been identified, which activates the abscission checkpoint in response to chromatin bridges, preventing chromosome breakage or tetraploidization [1,2].
During cell division, inhibition of ATM or Chk2 kinases impairs CPC localization to the midbody center, accelerates midbody resolution, and leads to premature abscission and chromatin breakage in cytokinesis with trapped chromatin. In cultured human cells, ATM activates Chk2 at late midbodies, and Chk2 phosphorylates human Incenp-Ser91. This phosphorylation promotes Incenp binding to Mklp2 kinesin and CPC localization to the midbody center through Mklp2 association with Cep55 [1]. Expression of truncated Mklp2 that does not bind to Cep55 or non-phosphorylatable Incenp-Ser91A impairs CPC midbody localization and accelerates abscission, while phosphomimetic Incenp-Ser91D or a chimeric Incenp protein targeted to the midbody center rescues the abscission delay in Chk2-deficient or ATM-deficient cells [1].
In conclusion, Incenp is essential for proper chromosome segregation and regulation of cell division processes. The ATM-Chk2-Incenp pathway, as revealed through functional studies, plays a vital role in preventing chromatin breakage during cytokinesis. Understanding Incenp's functions can provide insights into the mechanisms underlying normal cell division and potentially shed light on diseases related to abnormal cell division, such as cancer.
References:
1. Petsalaki, Eleni, Zachos, George. . An ATM-Chk2-INCENP pathway activates the abscission checkpoint. In The Journal of cell biology, 220, . doi:10.1083/jcb.202008029. https://pubmed.ncbi.nlm.nih.gov/33355621/
2. Petsalaki, Eleni, Zachos, George. 2021. An ATM-CHK2-INCENP pathway prevents chromatin breakage by regulating the abscission checkpoint. In Molecular & cellular oncology, 8, 1877999. doi:10.1080/23723556.2021.1877999. https://pubmed.ncbi.nlm.nih.gov/33860082/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen