C57BL/6JCya-Tmem150aem1/Cya
Common Name:
Tmem150a-KO
Product ID:
S-KO-17926
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmem150a-KO
Strain ID
KOCMP-232086-Tmem150a-B6J-VB
Gene Name
Product ID
S-KO-17926
Gene Alias
Tmem150
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem150aem1/Cya mice (Catalog S-KO-17926) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069695
NCBI RefSeq
NM_144916
Target Region
Exon 3~7
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Tmem150a, also known as TM6P1 and damage-regulated autophagy modulator 5, is a transmembrane protein that plays a crucial role in regulating phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] production at the plasma membrane. It modifies the composition of the phosphatidylinositol 4-kinase enzyme complex, and this regulation is part of the Toll-like receptor 4 (TLR4) signaling pathway, which is important for the body's immune response [1,3,4].
Knockdown of Tmem150a in TLR4-expressing epithelial cells led to increased levels of lipopolysaccharide (LPS)-induced cytokine secretion and transcript levels. In a lung epithelial cell line (H292), Tmem150a knockdown also increased cytokine levels in unstimulated conditions, suggesting its role in cellular homeostasis [1]. In glioblastoma multiforme (GBM), overexpression of Tmem150a was observed in cancerous tissues. Suppressing its expression inhibited GBM cell proliferation, migration, and invasion. Also, Tmem150a overexpression was associated with stromal, immune, and estimate scores, immune cells, cell markers, and RNA modifications, indicating its potential as a biomarker for poor prognosis in GBM [2].
In conclusion, Tmem150a is essential for maintaining PI(4,5)P2 homeostasis and cellular cytokine regulation. Gene knockdown models have revealed its significance in immune-related cytokine regulation and in the context of glioblastoma multiforme. Understanding Tmem150a function through these models provides insights into disease mechanisms and potential therapeutic targets for diseases such as GBM.
References:
1. Romanet, Jessica L, Cupo, Katherine L, Yoder, Jeffrey A. . Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines. In Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 42, 336-342. doi:10.1089/jir.2022.0063. https://pubmed.ncbi.nlm.nih.gov/35834652/
2. Fan, Si-Tong, Xu, Hao-Qiang, He, Yang, Yang, Wen-Qiong, Qin, Yong. 2023. Overexpression of TMEM150A in glioblastoma multiforme patients correlated with dismal prognoses and compromised immune statuses. In PloS one, 18, e0294144. doi:10.1371/journal.pone.0294144. https://pubmed.ncbi.nlm.nih.gov/38055673/
3. Batrouni, Alex G, Bag, Nirmalya, Phan, Henry T, Baird, Barbara A, Baskin, Jeremy M. 2021. A palmitoylation code controls PI4KIIIα complex formation and PI(4,5)P2 homeostasis at the plasma membrane. In Journal of cell science, 135, . doi:10.1242/jcs.259365. https://pubmed.ncbi.nlm.nih.gov/34569608/
4. Chung, Jeeyun, Nakatsu, Fubito, Baskin, Jeremy M, De Camilli, Pietro. 2015. Plasticity of PI4KIIIα interactions at the plasma membrane. In EMBO reports, 16, 312-20. doi:10.15252/embr.201439151. https://pubmed.ncbi.nlm.nih.gov/25608530/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen