C57BL/6JCya-Pik3ap1em1/Cya
Common Name:
Pik3ap1-KO
Product ID:
S-KO-17928
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pik3ap1-KO
Strain ID
KOCMP-83490-Pik3ap1-B6J-VA
Gene Name
Product ID
S-KO-17928
Gene Alias
1810044J04Rik; BCAP
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pik3ap1em1/Cya mice (Catalog S-KO-17928) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059672
NCBI RefSeq
NM_031376
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Pik3ap1, also known as B-cell adapter for PI3K (BCAP), plays a crucial role in the PI3K/AKT signaling pathway [3,5,6,7,8]. It is involved in regulating various biological processes such as immune response, cell proliferation, and macrophage transition, which are vital for maintaining normal physiological functions [1,3,4,5,6,7,8,9].
In macrophage-specific BCAP-deficient mice, they fail to recover from dextran sulfate sodium-induced colitis due to prolonged intestinal inflammation and impaired tissue repair, indicating that Pik3ap1 is essential for the transition of macrophages from an inflammatory to a reparative state [1]. In patients with Periodic Fever, Aphthous Stomatitis, Pharyngitis, and Adenitis (PFAPA) syndrome, the PIK3AP1 gene region shows differential methylation, suggesting its potential role in the etiology of this auto-inflammatory disease [2]. Gastric epithelial cells (GES-1) stimulated by Helicobacter pylori show hypermethylation of the PIK3AP1 promoter, leading to down-regulation of PIK3AP1 expression. Intervention of PIK3AP1 inhibits the expression of downstream gene AKT and the immunoinflammatory gene IL-6, and promotes cell proliferation [3]. Overexpression of PIK3AP1 significantly inhibits African swine fever virus (ASFV) replication in vitro, and this suppressive effect is independent of the PI3K-Akt pathway [4]. In gastric cancer, PIK3AP1 activates AKT phosphorylation, and a miR-567-PIK3AP1-PI3K/AKT-c-Myc feedback loop regulates tumour growth and chemoresistance [5]. In thyroid cancer, miR-1246 targets PIK3AP1 to regulate the PI3K/AKT signaling pathway, inhibiting cell proliferation and tumor growth [6]. In cervical cancer, WT1-AS inhibits the progression of cervical cancer through the SPI1/PIK3AP1 axis [7]. In hepatocellular carcinoma, BRG1 regulates lipid metabolism through the PIK3AP1/PI3K/AKT pathway by mediating GLMP expression [8]. In Behcet's disease, patients with high disease activity have higher expression of PIK3AP1 [9].
In conclusion, Pik3ap1 is a key regulator in the PI3K/AKT signaling pathway, participating in numerous biological processes. Studies using gene-knockout or conditional-knockout mouse models, as well as research on various human diseases, have revealed its significant roles in inflammation, auto-inflammation, virus-host interactions, tumorigenesis, and lipid metabolism. Understanding Pik3ap1 function provides insights into the underlying mechanisms of these biological processes and diseases, potentially guiding the development of new therapeutic strategies.
References:
1. Irizarry-Caro, Ricardo A, McDaniel, Margaret M, Overcast, Garrett R, Troutman, Ty Dale, Pasare, Chandrashekhar. 2020. TLR signaling adapter BCAP regulates inflammatory to reparatory macrophage transition by promoting histone lactylation. In Proceedings of the National Academy of Sciences of the United States of America, 117, 30628-30638. doi:10.1073/pnas.2009778117. https://pubmed.ncbi.nlm.nih.gov/33199625/
2. Lovšin, Ema, Kovač, Jernej, Tesovnik, Tine, Debeljak, Maruša, Avčin, Tadej. 2020. PIK3AP1 and SPON2 Genes Are Differentially Methylated in Patients With Periodic Fever, Aphthous Stomatitis, Pharyngitis, and Adenitis (PFAPA) Syndrome. In Frontiers in immunology, 11, 1322. doi:10.3389/fimmu.2020.01322. https://pubmed.ncbi.nlm.nih.gov/32793186/
3. Zhang, Andong, Yan, Shiying, Cao, Mei, Hu, Guoku, Zhao, Jian. 2020. Abnormal methylation of PIK3AP1 was involved in regulating the immune inflammatory response of GES-1 cells induced by Helicobacter pylori. In Biochemical and biophysical research communications, 524, 36-42. doi:10.1016/j.bbrc.2020.01.007. https://pubmed.ncbi.nlm.nih.gov/31980170/
4. Yang, Bo, Hao, Yu, Yang, Jinke, Zheng, Haixue, Zhang, Keshan. 2023. PI3K-Akt pathway-independent PIK3AP1 identified as a replication inhibitor of the African swine fever virus based on iTRAQ proteomic analysis. In Virus research, 327, 199052. doi:10.1016/j.virusres.2023.199052. https://pubmed.ncbi.nlm.nih.gov/36775023/
5. Zhang, Feifei, Li, Kaitao, Yao, Xueqing, Xu, Lijun, Zhao, Liang. 2019. A miR-567-PIK3AP1-PI3K/AKT-c-Myc feedback loop regulates tumour growth and chemoresistance in gastric cancer. In EBioMedicine, 44, 311-321. doi:10.1016/j.ebiom.2019.05.003. https://pubmed.ncbi.nlm.nih.gov/31078520/
6. Li, Jingyan, Zhang, Zhanlei, Hu, Jieting, Zhang, Hong, Jiang, Ningyi. 2021. MiR-1246 regulates the PI3K/AKT signaling pathway by targeting PIK3AP1 and inhibits thyroid cancer cell proliferation and tumor growth. In Molecular and cellular biochemistry, 477, 649-661. doi:10.1007/s11010-021-04290-3. https://pubmed.ncbi.nlm.nih.gov/34870753/
7. Tong, Wenjuan, Zhang, Huiming. 2021. Overexpression of long non-coding RNA WT1-AS or silencing of PIK3AP1 are inhibitory to cervical cancer progression. In Cell cycle (Georgetown, Tex.), 20, 2583-2596. doi:10.1080/15384101.2021.1991106. https://pubmed.ncbi.nlm.nih.gov/34839795/
8. Liu, Gao, Sun, Bao-Ye, Sun, Jian, Yi, Yong, Qiu, Shuang-Jian. 2021. BRG1 regulates lipid metabolism in hepatocellular carcinoma through the PIK3AP1/PI3K/AKT pathway by mediating GLMP expression. In Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 54, 692-700. doi:10.1016/j.dld.2021.05.002. https://pubmed.ncbi.nlm.nih.gov/34158256/
9. Liu, Huan, Zhang, Panpan, Li, Fuzhen, Du, Liping, Yang, Peizeng. 2023. Identification of the immune-related biomarkers in Behcet's disease by plasma proteomic analysis. In Arthritis research & therapy, 25, 92. doi:10.1186/s13075-023-03074-y. https://pubmed.ncbi.nlm.nih.gov/37264476/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen