C57BL/6JCya-Uchl3em1/Cya
Common Name:
Uchl3-KO
Product ID:
S-KO-17957
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Uchl3-KO
Strain ID
KOCMP-50933-Uchl3-B6J-VA
Gene Name
Product ID
S-KO-17957
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Uchl3em1/Cya mice (Catalog S-KO-17957) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002289
NCBI RefSeq
NM_016723
Target Region
Exon 3~6
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
UCHL3, also known as ubiquitin C-terminal hydrolase 3, is a cysteine protease that conducts deubiquitination and deneddylation activities. It plays a crucial role in cell cycle regulation, DNA repair, and apoptosis, and is involved in maintaining protein homeostasis within cells. Dysregulation of UCHL3 has been associated with neurodegenerative diseases, and it has emerged as a promising therapeutic target for certain cancers due to its ability to stabilize oncoproteins [2].
UCHL3 depletion significantly decreased anaplastic thyroid cancer (ATC) progression, stem-like and metastasis, and increased cell sensitivity to chemotherapy. It was found that UCHL3 stabilizes YAP in ATC in a deubiquitylation activity-dependent manner, and TEAD4 activates UCHL3 transcription by binding to its promoter [1]. In hepatocellular carcinoma (HCC), UCHL3 binds to and stabilizes EEF1A1 through deubiquitination, promoting HCC cell migration, stemness, and drug resistance [3]. In glioma stem cells, targeted depletion of the UCHL3-POLD4 axis reduced self-renewal, tumorigenic capacity, and radiation resistance [4]. In bladder cancer, depletion of UCHL3 in cancer cells delayed tumorigenesis in vitro and in vivo, while its overexpression boosted cell proliferation, invasion, and migration [5]. In cervical cancer, knockdown of UCHL3 inhibited cell growth, migration, and invasion in vitro and cancer development and metastasis in vivo [6].
In conclusion, UCHL3 is essential for multiple cellular processes, especially in maintaining protein stability through deubiquitination. The use of UCHL3 knockout or conditional knockout mouse models has revealed its significant role in promoting the progression of various cancers, including ATC, HCC, glioblastoma, bladder cancer, and cervical cancer. These findings suggest that UCHL3 could be a potential therapeutic target for treating these malignancies.
References:
1. Tang, Jianing, Yang, Qian, Mao, Chao, Wu, Gaosong, Tao, Yongguang. 2023. The deubiquitinating enzyme UCHL3 promotes anaplastic thyroid cancer progression and metastasis through Hippo signaling pathway. In Cell death and differentiation, 30, 1247-1259. doi:10.1038/s41418-023-01134-z. https://pubmed.ncbi.nlm.nih.gov/36813921/
2. Lei, Hu, Xu, Hanzhang, Wu, Yingli. 2024. Role of UCHL3 in health and disease. In Biochemical and biophysical research communications, 734, 150626. doi:10.1016/j.bbrc.2024.150626. https://pubmed.ncbi.nlm.nih.gov/39226739/
3. Zhao, Jie, Huo, Qiang, Zhang, Ji, Hu, Xiaoge, Xu, Qiuran. 2024. UCHL3 promotes hepatocellular carcinoma progression by stabilizing EEF1A1 through deubiquitination. In Biology direct, 19, 53. doi:10.1186/s13062-024-00495-w. https://pubmed.ncbi.nlm.nih.gov/38965582/
4. Fan, Ligang, You, Hongtao, Jiang, Xiao, Xing, Wei, Shao, Naiyuan. 2024. UCHL3 induces radiation resistance and acquisition of mesenchymal phenotypes by deubiquitinating POLD4 in glioma stem cells. In Cellular and molecular life sciences : CMLS, 81, 247. doi:10.1007/s00018-024-05265-5. https://pubmed.ncbi.nlm.nih.gov/38829550/
5. Liu, Tao, Fan, Meng-Qi, Xie, Xiao-Xiao, Du, Run-Lei, Li, Shang-Ze. 2023. Activation of CTNNB1 by deubiquitinase UCHL3-mediated stabilization facilitates bladder cancer progression. In Journal of translational medicine, 21, 656. doi:10.1186/s12967-023-04311-3. https://pubmed.ncbi.nlm.nih.gov/37740194/
6. Zhang, Yuan, Liu, Jing-Bo, Liu, Jing, Liu, Hong-Li, Zhang, Jing. 2022. UCHL3 promotes cervical cancer development and metastasis by stabilizing NRF2 via deubiquitination. In Biochemical and biophysical research communications, 641, 132-138. doi:10.1016/j.bbrc.2022.11.065. https://pubmed.ncbi.nlm.nih.gov/36527747/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen