C57BL/6JCya-Supt5em1/Cya
Common Name:
Supt5-KO
Product ID:
S-KO-17958
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Supt5-KO
Strain ID
KOCMP-20924-Supt5-B6J-VB
Gene Name
Product ID
S-KO-17958
Gene Alias
Spt5; Supt5h
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Supt5em1/Cya mice (Catalog S-KO-17958) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003527
NCBI RefSeq
NM_013676
Target Region
Exon 14~16
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Supt5, an ortholog of yeast transcriptional elongation factor Spt5, is involved in transcriptional elongation processes. It likely plays a significant role in maintaining normal cellular functions through its impact on gene expression regulation within various biological pathways [1].
In a study focusing on spinocerebellar ataxia type 36 (SCA36), small interfering RNA-mediated silencing of Supt5 (along with Supt4a, its murine ortholog) was investigated. The knockdown of Supt4a, related to Supt5, suppressed the formation of (GGCCUG)exp RNA foci and DPR protein formation via regulation of (GGCCUG)exp mRNA, thereby reducing cytotoxicity in SCA36 cell models [1].
In conclusion, Supt5 is crucial for transcriptional elongation and thus normal biological functions. The study of Supt5 knockdown in SCA36 cell models reveals its potential as a therapeutic target for SCA36 and other repeat-expansion disorders, highlighting the importance of such model-based research in understanding disease mechanisms and developing treatment strategies [1].
References：
1. Furuta, Natsumi, Tsukagoshi, Setsuki, Hirayanagi, Kimitoshi, Ikeda, Yoshio. 2019. Suppression of the yeast elongation factor Spt4 ortholog reduces expanded SCA36 GGCCUG repeat aggregation and cytotoxicity. In Brain research, 1711, 29-40. doi:10.1016/j.brainres.2018.12.045. https://pubmed.ncbi.nlm.nih.gov/30610877/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen