C57BL/6JCya-Syt12em1/Cya
Common Name:
Syt12-KO
Product ID:
S-KO-17976
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Syt12-KO
Strain ID
KOCMP-171180-Syt12-B6J-VB
Gene Name
Product ID
S-KO-17976
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Syt12em1/Cya mice (Catalog S-KO-17976) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059295
NCBI RefSeq
NM_134164
Target Region
Exon 4~6
Size of Effective Region
~4.6 kb
Detailed Document
Overview of Gene Research
Syt12, or Synaptotagmin 12, is a member of the synaptotagmins family. It is an abundant synaptic vesicle protein not binding Ca(2+), and is phosphorylated by cAMP-dependent protein kinase-A at serine-97 in an activity-dependent manner, suggesting its involvement in cAMP-dependent synaptic plasticity [5].
In cancer research, Syt12 has been found to play oncogenic roles. In thyroid carcinoma, it is significantly overexpressed, promoting carcinoma progression and metastasis, and is a potential biomarker for diagnosis and treatment in papillary thyroid cancer (PTC) [1]. In oral squamous cell carcinoma (OSCC), Syt12 is up-regulated, and its knockdown leads to depressed cellular proliferation, migration, and invasion with cell cycle arrest at G1 phase, suggesting its significance in OSCC progression [2]. In gastric cancer, it may promote the proliferation and migration of gastric cancer cells by inducing epithelial-mesenchymal transition (EMT), and is associated with clinicopathological features, immune cell infiltration, and prognosis [3]. In lung adenocarcinoma, its expression is increased, associated with advanced tumor stage and reduced prognosis, and it promotes cell proliferation and migration via the PI3K/AKT/mTOR pathway [4].
In conclusion, Syt12 is essential for cAMP-dependent presynaptic long-term potentiation (LTP) at mossy-fiber synapses in the hippocampus. In cancer, Syt12 acts as an oncogene promoting the progression of multiple cancers including thyroid, oral, gastric, and lung adenocarcinoma, highlighting its potential as a biomarker and therapeutic target in these diseases.
References:
1. Jin, Lingli, Zheng, Danni, Chen, Danxiang, Bhandari, Adheesh, Wang, Ouchen. 2021. SYT12 is a novel oncogene that promotes thyroid carcinoma progression and metastasis. In Journal of Cancer, 12, 6851-6860. doi:10.7150/jca.62555. https://pubmed.ncbi.nlm.nih.gov/34659573/
2. Eizuka, Keitaro, Nakashima, Dai, Oka, Noritoshi, Tanzawa, Hideki, Uzawa, Katsuhiro. 2019. SYT12 plays a critical role in oral cancer and may be a novel therapeutic target. In Journal of Cancer, 10, 4913-4920. doi:10.7150/jca.32582. https://pubmed.ncbi.nlm.nih.gov/31598163/
3. Niu, Xingdong, Ma, Fubin, Li, Fangying, Wang, Junhong, Da, Mingxu. 2024. Integration of bioinformatics and cellular experiments unveils the role of SYT12 in gastric cancer. In BMC cancer, 24, 1331. doi:10.1186/s12885-024-13077-w. https://pubmed.ncbi.nlm.nih.gov/39472897/
4. Liu, Kaichao, Luo, Jing, Shao, Chenye, Wu, Haiwei, Shen, Yi. 2020. Synaptotagmin 12 (SYT12) Gene Expression Promotes Cell Proliferation and Progression of Lung Adenocarcinoma and Involves the Phosphoinositide 3-Kinase (PI3K)/AKT/Mammalian Target of Rapamycin (mTOR) Pathway. In Medical science monitor : international medical journal of experimental and clinical research, 26, e920351. doi:10.12659/MSM.920351. https://pubmed.ncbi.nlm.nih.gov/32108133/
5. Kaeser-Woo, Yea Jin, Younts, Thomas J, Yang, Xiaofei, Castillo, Pablo E, Südhof, Thomas C. . Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 33, 9769-80. doi:10.1523/JNEUROSCI.5814-12.2013. https://pubmed.ncbi.nlm.nih.gov/23739973/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen