C57BL/6JCya-Sh3bgrlem1/Cya
Common Name:
Sh3bgrl-KO
Product ID:
S-KO-17994
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sh3bgrl-KO
Strain ID
KOCMP-56726-Sh3bgrl-B6J-VB
Gene Name
Product ID
S-KO-17994
Gene Alias
1190008F14Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sh3bgrlem1/Cya mice (Catalog S-KO-17994) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033598
NCBI RefSeq
NM_019989
Target Region
Exon 2~3
Size of Effective Region
~5.8 kb
Detailed Document
Overview of Gene Research
Sh3bgrl, also known as SH3 domain binding glutamate-rich protein-like, is an adaptor protein. It is involved in multiple biological processes. In cancer-related pathways, it impacts autophagy, cell cycle regulation, and signaling pathways such as Src-related ERK and AKT, HER2-related signaling, and AKT, NF-κB, and WNT pathways. In female reproduction, it is crucial for normal ovarian function [6].
In breast cancer, Sh3bgrl upregulation enhances chemoresistance to doxorubicin through promoting PIK3C3 translation and ATG12 stability, driving autophagy-mediated protection [1]. It also promotes breast cancer metastasis by downregulating PFN1 via translational STUB1 upregulation [3].
In liver cancer, Sh3bgrl overexpression inhibits cell proliferation and tumorigenesis by enhancing ATG5-dependent autophagy [2].
In acute myeloid leukemia, it is down-regulated, and its down-regulation promotes cell cycle progression and anti-apoptotic ability, while ectopic expression blocks cell cycle and sensitizes cells to drugs [4].
In lung cancer, it is down-regulated in cancer tissues and CSCs, and overexpression suppresses CSCs self-renewal [5].
In zebrafish, loss of Sh3bgrl leads to ovarian fibrosis and female spawning inability due to upregulation of ECM-associated genes [6].
In conclusion, Sh3bgrl plays diverse and significant roles in cancer development and female reproduction. Studies, including those using loss-of-function models, have revealed its functions in promoting or suppressing tumorigenesis in different cancers and its importance in maintaining normal ovarian function, providing potential diagnostic and therapeutic targets for related diseases.
References:
1. Zhang, Shaoyang, Liu, Xiufeng, Abdulmomen Ali Mohammed, Saleh, Yang, Shulan, Wang, Haihe. 2021. Adaptor SH3BGRL drives autophagy-mediated chemoresistance through promoting PIK3C3 translation and ATG12 stability in breast cancers. In Autophagy, 18, 1822-1840. doi:10.1080/15548627.2021.2002108. https://pubmed.ncbi.nlm.nih.gov/34870550/
2. Saleh, Abdulmomen Ali Mohammed, Haider, Farhan, Lv, Haimei, Yang, Shulan, Wang, Haihe. 2023. SH3BGRL Suppresses Liver Tumor Progression through Enhanced ATG5-Dependent Autophagy. In Journal of oncology, 2023, 1105042. doi:10.1155/2023/1105042. https://pubmed.ncbi.nlm.nih.gov/37138798/
3. Zhang, Shaoyang, Guo, Xuemin, Liu, Xiufeng, Yang, Shulan, Wang, Haihe. 2021. Adaptor SH3BGRL promotes breast cancer metastasis through PFN1 degradation by translational STUB1 upregulation. In Oncogene, 40, 5677-5690. doi:10.1038/s41388-021-01970-8. https://pubmed.ncbi.nlm.nih.gov/34331014/
4. Xu, Limei, Zhang, Mingming, Li, Hui, Tong, Xiuzhen, Wang, Haihe. 2017. SH3BGRL as a novel prognostic biomarker is down-regulated in acute myeloid leukemia. In Leukemia & lymphoma, 59, 918-930. doi:10.1080/10428194.2017.1344843. https://pubmed.ncbi.nlm.nih.gov/28679293/
5. Liu, Zhiping, Xu, Wen, Tan, Xiansheng, Li, Chunhua. 2021. EZH2-mediated epigenetic suppression of SH3BGRL potently inhibits lung cancer progression. In Biochemical and biophysical research communications, 548, 53-59. doi:10.1016/j.bbrc.2021.02.056. https://pubmed.ncbi.nlm.nih.gov/33631674/
6. Jin, Ziwei, Wang, Dongxia, Lv, Haimei, Wang, Haihe, Yang, Shulan. 2023. Loss of the adaptor protein Sh3bgrl initiates ovarian fibrosis in zebrafish. In FEBS letters, 597, 2643-2655. doi:10.1002/1873-3468.14733. https://pubmed.ncbi.nlm.nih.gov/37698355/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen