C57BL/6JCya-Xrn2em1/Cya
Common Name:
Xrn2-KO
Product ID:
S-KO-18000
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Xrn2-KO
Strain ID
KOCMP-24128-Xrn2-B6J-VB
Gene Name
Product ID
S-KO-18000
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Xrn2em1/Cya mice (Catalog S-KO-18000) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028921
NCBI RefSeq
NM_011917
Target Region
Exon 5~6
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
Xrn2 is a 5'-to-3' exoribonuclease predominantly located in the nucleus. By degrading or trimming various RNA classes, it contributes to essential gene expression processes like transcription termination and ribosome biogenesis. It also plays roles in DNA repair, replication stress resolution, and telomere stability [1,3,5,6,7,8].
Loss of Xrn2 in glioblastoma cell lines decreases cellular speed, displacement, and invasion, and abolishes tumor formation in orthotopic mouse xenografts, indicating its importance in glioblastoma invasion [2]. Xrn2 -deficient fibroblast and lung cancer cells show sensitivity to PARP1 inhibition, uncovering a synthetic lethal relationship [3]. In Brca1 -mutant breast cancer cells, RNF8 deficiency decreases XRN2 occupancy at R-loop-prone sites, promoting R-loop accumulation and cancer cell death [4]. Xrn2-deficient cells have increased R-loops, genomic instability, replication stress, and DSBs, and exhibit a delay in DSB repair after ionizing radiation [7]. Depletion of XRN2 in ALT-positive cancer cells leads to increased TERRA R-loops and exacerbated ALT activity [8].
In conclusion, Xrn2 is crucial for multiple biological processes including transcription, DNA repair, and telomere stability. Studies using gene knockout models in cancer cell lines and mouse xenografts have revealed its significance in glioblastoma invasion, synthetic lethality with PARP1 inhibition, and telomere metabolism in ALT-positive cancer cells, providing potential therapeutic targets for these diseases.
References:
1. Aygün, Ilkin, Miki, Takashi S. 2021. Nuclear RNA Regulation by XRN2 and XTBD Family Proteins. In Cell structure and function, 46, 73-78. doi:10.1247/csf.21041. https://pubmed.ncbi.nlm.nih.gov/34483148/
2. Dang, Tuyen T, Lerner, Megan, Saunders, Debra, Towner, Rheal A, Morales, Julio C. 2022. XRN2 Is Required for Cell Motility and Invasion in Glioblastomas. In Cells, 11, . doi:10.3390/cells11091481. https://pubmed.ncbi.nlm.nih.gov/35563787/
3. Patidar, Praveen L, Viera, Talysa, Morales, Julio C, Khandelwal, Megha, Fattah, Farjana J. 2020. XRN2 interactome reveals its synthetic lethal relationship with PARP1 inhibition. In Scientific reports, 10, 14253. doi:10.1038/s41598-020-71203-7. https://pubmed.ncbi.nlm.nih.gov/32859985/
4. Krishnan, Rehna, Lapierre, Mariah, Gautreau, Brandon, Hakem, Anne, Hakem, Razqallah. . RNF8 ubiquitylation of XRN2 facilitates R-loop resolution and restrains genomic instability in BRCA1 mutant cells. In Nucleic acids research, 51, 10484-10505. doi:10.1093/nar/gkad733. https://pubmed.ncbi.nlm.nih.gov/37697435/
5. Miki, Takashi S, Großhans, Helge. . The multifunctional RNase XRN2. In Biochemical Society transactions, 41, 825-30. doi:10.1042/BST20130001. https://pubmed.ncbi.nlm.nih.gov/23863139/
6. Dang, Tuyen T, Morales, Julio C. 2020. XRN2 Links RNA:DNA Hybrid Resolution to Double Strand Break Repair Pathway Choice. In Cancers, 12, . doi:10.3390/cancers12071821. https://pubmed.ncbi.nlm.nih.gov/32645903/
7. Morales, Julio C, Richard, Patricia, Patidar, Praveen L, Manley, James L, Boothman, David A. 2016. XRN2 Links Transcription Termination to DNA Damage and Replication Stress. In PLoS genetics, 12, e1006107. doi:10.1371/journal.pgen.1006107. https://pubmed.ncbi.nlm.nih.gov/27437695/
8. Reiss, Matthew, Keegan, Joshua, Aldrich, Anne, Lyons, Shawn M, Flynn, Rachel Litman. 2023. The exoribonuclease XRN2 mediates degradation of the long non-coding telomeric RNA TERRA. In FEBS letters, 597, 1818-1836. doi:10.1002/1873-3468.14639. https://pubmed.ncbi.nlm.nih.gov/37191774/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen