C57BL/6JCya-Rnf20em1/Cya
Common Name
Rnf20-KO
Product ID
S-KO-18011
Backgroud
C57BL/6JCya
Strain ID
KOCMP-109331-Rnf20-B6J-VB
When using this mouse strain in a publication, please cite “Rnf20-KO Mouse (Catalog S-KO-18011) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Rnf20-KO
Strain ID
KOCMP-109331-Rnf20-B6J-VB
Gene Name
Product ID
S-KO-18011
Gene Alias
4833430L21Rik, mKIAA4116
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 4
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000167496
NCBI RefSeq
NM_001163263
Target Region
Exon 3~5
Size of Effective Region
~1.8 kb
Overview of Gene Research
RNF20, the human homolog of yeast ubiquitin ligase Bre1, is an E3 ubiquitin ligase crucial for histone H2B monoubiquitination (H2Bub) [1,3,5,6]. It participates in multiple biological pathways, including homologous recombination, epigenetic regulation, and transcriptional control, thus playing an important role in maintaining genome stability, cell differentiation, and organismal development [1,2,3,4,5,6]. Genetic models, such as gene knockout (KO) or conditional knockout (CKO) mouse models, have been instrumental in understanding its functions.
In KO mouse models, adipocyte-specific Rnf20 knockout led to progressive fat loss, organomegaly, hyperinsulinemia, and impaired adipose tissue development and thermogenesis, suggesting RNF20's role in adipogenesis, at least in part, through regulating peroxisome proliferator-activated receptor gamma (Pparγ) [8]. RNF20 deletion in mice caused increased inflammation reactions in a sepsis model, indicating its role in suppressing inflammation via the NLRP3 inflammasome and NLRP3 ubiquitination by activating Vitamin D Receptor (VDR) [7].
In conclusion, RNF20 is essential for multiple biological functions, including homologous recombination, epigenetic regulation, and transcriptional control. Its deletion in KO/CKO mouse models reveals its significance in diseases like sepsis and adipose-related disorders, providing insights into the underlying disease mechanisms and potential therapeutic targets.
References：
1. Liu, Guangxue, Li, Jimin, He, Boxue, Zhang, Xinghua, Chen, Xuefeng. 2023. Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases. In Nature communications, 14, 3024. doi:10.1038/s41467-023-38617-z. https://pubmed.ncbi.nlm.nih.gov/37230987/
2. Dong, Bo, Wang, Xinzhao, Song, Xiang, Deng, Jiong, Wu, Yadi. 2024. RNF20 contributes to epigenetic immunosuppression through CDK9-dependent LSD1 stabilization. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2307150121. doi:10.1073/pnas.2307150121. https://pubmed.ncbi.nlm.nih.gov/38315842/
3. Li, Jimin, Zhao, Jingyu, Gan, Xiaoli, Huang, Jun, Chen, Xuefeng. 2023. The RPA-RNF20-SNF2H cascade promotes proper chromosome segregation and homologous recombination repair. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2303479120. doi:10.1073/pnas.2303479120. https://pubmed.ncbi.nlm.nih.gov/37155876/
4. Tetik-Elsherbiny, Nalan, Elsherbiny, Adel, Setya, Aadhyaa, Ola, Roxana, Dobreva, Gergana. 2024. RNF20-mediated transcriptional pausing and VEGFA splicing orchestrate vessel growth. In Nature cardiovascular research, 3, 1199-1216. doi:10.1038/s44161-024-00546-5. https://pubmed.ncbi.nlm.nih.gov/39322771/
5. Kato, Akihiro, Komatsu, Kenshi. 2015. RNF20-SNF2H Pathway of Chromatin Relaxation in DNA Double-Strand Break Repair. In Genes, 6, 592-606. doi:10.3390/genes6030592. https://pubmed.ncbi.nlm.nih.gov/26184323/
6. In, Suna, Kim, Yong-In, Lee, J Eugene, Kim, Jaehoon. . RNF20/40-mediated eEF1BδL monoubiquitylation stimulates transcription of heat shock-responsive genes. In Nucleic acids research, 47, 2840-2855. doi:10.1093/nar/gkz006. https://pubmed.ncbi.nlm.nih.gov/30649429/
7. Qi, Anlong, Liu, Yancun, Zhai, Jianhua, Wang, Tong, Chai, Yanfen. 2023. RNF20 deletion causes inflammation in model of sepsis through the NLRP3 activation. In Immunopharmacology and immunotoxicology, 45, 469-478. doi:10.1080/08923973.2023.2170241. https://pubmed.ncbi.nlm.nih.gov/36650938/
8. Liang, Xiaojuan, Tao, Cong, Pan, Jianfei, Li, Kui, Wang, Yanfang. 2020. Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis. In Protein & cell, 12, 475-492. doi:10.1007/s13238-020-00770-2. https://pubmed.ncbi.nlm.nih.gov/32797353/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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