C57BL/6JCya-Glycam1em1/Cya
Common Name
Glycam1-KO
Product ID
S-KO-18013
Backgroud
C57BL/6JCya
Strain ID
KOCMP-14663-Glycam1-B6J-VA
When using this mouse strain in a publication, please cite “Glycam1-KO Mouse (Catalog S-KO-18013) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Glycam1-KO
Strain ID
KOCMP-14663-Glycam1-B6J-VA
Gene Name
Product ID
S-KO-18013
Gene Alias
MC26, Sgp50, glyCAM-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000023134
NCBI RefSeq
NM_008134
Target Region
Exon 2~4
Size of Effective Region
~2.5 kb
Overview of Gene Research
Glycam1, short for Glycosylation-dependent Cell Adhesion Molecule 1, is a member of the glycoprotein mucin family. It encodes a proteoglycan ligand for L-selectin, which is involved in modulating immune cell entry, and is a component of the milk fat globule membrane (MFGM) [2,4]. It may play roles in lymphocyte trafficking and could be associated with processes like inflammation, immunity, and mammary gland function [2,3,4]. Genetic models such as knockout mice can be valuable for studying its functions.
In a DBA/2J mouse model of glaucoma, radiation-induced over-expression of Glycam1 was found to be part of the neuroprotective mechanism. Mice with a null allele of Glycam1 showed increased monocyte entry into the optic nerve head, as well as increased retinal ganglion cell death and axon loss after radiation therapy, although radiation still provided some protection. This indicates that Glycam1 negatively regulates monocyte entry into the optic nerve head and contributes to radiation-based protection in glaucoma [1].
In conclusion, Glycam1 functions as a negative regulator of extravasation, modulating immune cell entry into neural tissues, which is demonstrated in the glaucoma mouse model. Its role in radiation-mediated protection in glaucoma shows that Glycam1-related research may lead to novel therapeutic strategies for conditions involving inflammation [1].
References：
1. Williams, Pete A, Braine, Catherine E, Foxworth, Nicole E, Cochran, Kelly E, John, Simon W M. 2017. GlyCAM1 negatively regulates monocyte entry into the optic nerve head and contributes to radiation-based protection in glaucoma. In Journal of neuroinflammation, 14, 93. doi:10.1186/s12974-017-0868-8. https://pubmed.ncbi.nlm.nih.gov/28446179/
2. Le Provost, Fabienne, Cassy, Sandrine, Hayes, Hélène, Martin, Patrice. . Structure and expression of goat GLYCAM1 gene: lactogenic-dependent expression in ruminant mammary gland and interspecies conservation of the proximal promoter. In Gene, 313, 83-9. doi:. https://pubmed.ncbi.nlm.nih.gov/12957379/
3. Dill-Garlow, Riva, Chen, KuanHui Ethan, Walker, Ameae M. 2019. Sex Differences in Mouse Popliteal Lymph Nodes. In Scientific reports, 9, 965. doi:10.1038/s41598-018-37175-5. https://pubmed.ncbi.nlm.nih.gov/30700819/
4. Nishimura, Toshihide. . Expression of potential lymphocyte trafficking mediator molecules in the mammary gland. In Veterinary research, 34, 3-10. doi:. https://pubmed.ncbi.nlm.nih.gov/12588680/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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