C57BL/6JCya-Il33em1/Cya
Common Name:
Il33-KO
Product ID:
S-KO-18022
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Il33-KO
Strain ID
KOCMP-77125-Il33-B6J-VB
Gene Name
Product ID
S-KO-18022
Gene Alias
9230117N10Rik; Il-33; Il1f11; NF-HEV
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il33em1/Cya mice (Catalog S-KO-18022) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025724
NCBI RefSeq
NM_001164724.2
Target Region
Exon 3~7
Size of Effective Region
~6.3 kb
Detailed Document
Overview of Gene Research
Interleukin-33 (IL33) belongs to the IL-1 cytokine family. It is constitutively produced by structural and lining cells such as fibroblasts, endothelial cells, and epithelial cells of various tissues [1]. Unlike most cytokines, it is a nuclear cytokine that is passively released during cell necrosis or tissue damage, acting as an alarmin to alert the immune system [1]. IL33 plays crucial roles in type-2 innate immunity by activating eosinophils, basophils, mast cells, macrophages, and group 2 innate lymphoid cells (ILC2s) via its receptor ST2 [1]. It is involved in various inflammatory, infectious, and autoimmune diseases [3].
In mouse models of spontaneous breast cancer metastasis, targeting IL33 in vivo inhibited lung metastasis and attenuated immune cell recruitment and type 2 immunity, suggesting its role in facilitating breast cancer lung metastasis by modifying the immune microenvironment [2]. In pancreatic cancer mouse models, lack of stromal IL33 led to reprogramming of the tumor microenvironment components like CAFs, myeloid cells, and lymphocytes, along with increased CD8+ T-cell infiltration and reduced tumor growth, indicating its role in promoting pancreatic tumor growth [4].
In conclusion, IL33 is a key cytokine in immune regulation and inflammation, playing important roles in diseases such as allergic diseases, breast cancer, and pancreatic cancer. The use of gene knockout (KO) and conditional knockout (CKO) mouse models has significantly contributed to understanding IL33's functions in these disease conditions, providing insights into potential therapeutic targets.
References:
1. Chan, Ben C L, Lam, Christopher W K, Tam, Lai-Shan, Wong, Chun K. 2019. IL33: Roles in Allergic Inflammation and Therapeutic Perspectives. In Frontiers in immunology, 10, 364. doi:10.3389/fimmu.2019.00364. https://pubmed.ncbi.nlm.nih.gov/30886621/
2. Shani, Ophir, Vorobyov, Tatiana, Monteran, Lea, Barshack, Iris, Erez, Neta. 2020. Fibroblast-Derived IL33 Facilitates Breast Cancer Metastasis by Modifying the Immune Microenvironment and Driving Type 2 Immunity. In Cancer research, 80, 5317-5329. doi:10.1158/0008-5472.CAN-20-2116. https://pubmed.ncbi.nlm.nih.gov/33023944/
3. Tonacci, Alessandro, Quattrocchi, Paolina, Gangemi, Sebastiano. 2019. IL33/ST2 Axis in Diabetic Kidney Disease: A Literature Review. In Medicina (Kaunas, Lithuania), 55, . doi:10.3390/medicina55020050. https://pubmed.ncbi.nlm.nih.gov/30769901/
4. Donahue, Katelyn L, Watkoske, Hannah R, Kadiyala, Padma, Zhang, Yaqing, Pasca di Magliano, Marina. . Oncogenic KRAS-Dependent Stromal Interleukin-33 Directs the Pancreatic Microenvironment to Promote Tumor Growth. In Cancer discovery, 14, 1964-1989. doi:10.1158/2159-8290.CD-24-0100. https://pubmed.ncbi.nlm.nih.gov/38958646/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen