C57BL/6JCya-Dusp4em1/Cya
Common Name
Dusp4-KO
Product ID
S-KO-18027
Backgroud
C57BL/6JCya
Strain ID
KOCMP-319520-Dusp4-B6J-VB
When using this mouse strain in a publication, please cite “Dusp4-KO Mouse (Catalog S-KO-18027) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Dusp4-KO
Strain ID
KOCMP-319520-Dusp4-B6J-VB
Gene Name
Product ID
S-KO-18027
Gene Alias
2700078F24Rik, E130306H24Rik, MKP2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000033930
NCBI RefSeq
NM_176933
Target Region
Exon 2~4
Size of Effective Region
~4.2 kb
Overview of Gene Research
DUSP4, a dual-specificity phosphatase, is a key regulator in multiple biological pathways. It inactivates factors in the mitogen-activated protein kinase (MAPK) signaling cascade, such as extracellular signal-regulated kinase (ERK) 1/2 [3,4,5,6,8]. It also plays roles in innate immune signaling, regulating the activation of TBK1 and ERK1/2 in a complex with IRF3 to control type I interferon production [1]. Genetic models like KO mouse models are valuable for studying its functions.
In KO mouse models, DUSP4-deficient mice were more resistant to RNA and DNA virus infections but more susceptible to malaria parasites, indicating its role in nucleic acid sensor signaling and type I IFN regulation [1]. In esophageal squamous cell carcinoma, DUSP4 knockout in mice significantly inhibited 4-nitrochinoline-oxide-induced esophageal tumorigenesis, as DUSP4 promotes tumor progression by dephosphorylating HSP90β [2]. In endometroid carcinoma, ARID1A deficiency downregulates DUSP4, and ectopic DUSP4 expression decreased cell proliferation [4]. In clear cell renal cell carcinoma (CCRCC), DUSP4 silencing inhibited cell proliferation, migration, and tumorigenicity, and reversed autophagic activity changes induced by DUSP4 overexpression [7].
In conclusion, DUSP4 has diverse functions in regulating immune responses and cancer-related biological processes. The KO mouse models have been crucial in revealing its roles in virus-parasite resistance, esophageal and endometroid carcinoma, and CCRCC, providing insights into potential therapeutic targets for related diseases.
References：
1. Jiao, Huipeng, James, Sharmy J, Png, Chin Wen, Deng, Yinyue, Zhang, Yongliang. 2024. DUSP4 modulates RIG-I- and STING-mediated IRF3-type I IFN response. In Cell death and differentiation, 31, 280-291. doi:10.1038/s41418-024-01269-7. https://pubmed.ncbi.nlm.nih.gov/38383887/
2. Zhou, Liting, Yao, Ning, Yang, Lu, Dong, Zigang, Li, Xiang. 2023. DUSP4 promotes esophageal squamous cell carcinoma progression by dephosphorylating HSP90β. In Cell reports, 42, 112445. doi:10.1016/j.celrep.2023.112445. https://pubmed.ncbi.nlm.nih.gov/37141098/
3. Ito, Takahiro, Young, Michael J, Li, Ruitong, Zamanighomi, Mahdi, Sellers, William R. 2021. Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers. In Nature genetics, 53, 1664-1672. doi:10.1038/s41588-021-00967-z. https://pubmed.ncbi.nlm.nih.gov/34857952/
4. Mandal, Jayaprakash, Yu, Zheng-Cheng, Shih, Ie-Ming, Wang, Tian-Li. 2023. ARID1A loss activates MAPK signaling via DUSP4 downregulation. In Journal of biomedical science, 30, 94. doi:10.1186/s12929-023-00985-5. https://pubmed.ncbi.nlm.nih.gov/38071325/
5. Klemm, Sophie, Evert, Katja, Utpatel, Kirsten, Calvisi, Diego F, Scheiter, Alexander. 2023. Identification of DUSP4/6 overexpression as a potential rheostat to NRAS-induced hepatocarcinogenesis. In BMC cancer, 23, 1086. doi:10.1186/s12885-023-11577-9. https://pubmed.ncbi.nlm.nih.gov/37946160/
6. Aughton, Karen, Sabat-Pospiech, Dorota, Barlow, Samantha, Coupland, Sarah E, Kalirai, Helen. . Investigating the Role of DUSP4 in Uveal Melanoma. In Translational vision science & technology, 11, 13. doi:10.1167/tvst.11.12.13. https://pubmed.ncbi.nlm.nih.gov/36576731/
7. Zeng, Xianyou, Zhu, Changyan, Zhu, Xianxin. . DUSP4 promotes the carcinogenesis of CCRCC via negative regulation of autophagic death. In Bioscience, biotechnology, and biochemistry, 85, 1839-1845. doi:10.1093/bbb/zbab111. https://pubmed.ncbi.nlm.nih.gov/34143206/
8. Kamada, Hirofumi, Yasuhira, Shinji, Shibazaki, Masahiko, Amano, Hiroo, Maesawa, Chihaya. 2022. DUSP4 Inactivation Leads to Reduced Extracellular Signal‒Regulated Kinase Activity through Upregulation of DUSP6 in Melanoma Cells. In The Journal of investigative dermatology, 142, 2499-2507.e6. doi:10.1016/j.jid.2022.02.007. https://pubmed.ncbi.nlm.nih.gov/35189148/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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