C57BL/6JCya-Vps29em1/Cya
Common Name:
Vps29-KO
Product ID:
S-KO-18044
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Vps29-KO
Strain ID
KOCMP-56433-Vps29-B6J-VB
Gene Name
Product ID
S-KO-18044
Gene Alias
2010015D08Rik; PEP11
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vps29em1/Cya mice (Catalog S-KO-18044) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000118830
NCBI RefSeq
NM_001347453.2
Target Region
Exon 2~4
Size of Effective Region
~5478 bp
Detailed Document
Overview of Gene Research
Vps29, short for vacuolar protein sorting 29, is a key component of the retromer complex. The retromer complex is involved in the endosome-to-Golgi retrieval cargo transport pathway, and Vps29 plays a crucial role in coordinating the physical assembly of retrieval complexes with regulatory components, thus being essential for endosomal recycling [1,4,5,6,8]. This process is vital for maintaining the proteostatic plasticity of the cell under variable physiological demands [1].
In Drosophila, Vps29 is dispensable for embryogenesis but required for retromer function in aging adults. Vps29 mutants show mislocalization of retromer from neuropil to soma with the Rab7 GTPase, leading to progressive endolysosomal dysfunction, impaired substrate clearance, and lysosomal stress, highlighting its role in brain homeostasis during aging [6]. In human lung cell lines, a loss-of-function genome-wide CRISPR screen showed that Vps29 is required for the infection of several coronaviruses including SARS-CoV-2 and ebolavirus, while it has an enhancing effect on influenza A virus infection. Deficiency in Vps29 causes changes in endosome morphology and acidity and attenuates endosomal protease activity, entrapping incoming coronavirus particles [2]. In a human iPSC-derived neuronal model of 4R tauopathy, a CRISPRi screen identified Vps29 as a genetic modifier of seeding-induced Tau propagation [3]. A bioinformatics and Mendelian randomization study indicated that Vps29 is overexpressed in hepatocellular carcinoma (HCC), is an independent prognostic factor, and is associated with an increased risk of HCC [7].
In conclusion, Vps29 is integral to endosomal recycling, which is essential for normal cellular function. Model-based research, such as in Drosophila and human cell lines, has revealed its significance in various disease-related processes, including viral infections, neurodegenerative diseases like tauopathy, and cancer, specifically HCC. Understanding Vps29's function through these models provides insights into potential therapeutic targets for these diseases.
References：
1. Baños-Mateos, Soledad, Rojas, Adriana L, Hierro, Aitor. 2019. VPS29, a tweak tool of endosomal recycling. In Current opinion in cell biology, 59, 81-87. doi:10.1016/j.ceb.2019.03.010. https://pubmed.ncbi.nlm.nih.gov/31051431/
2. Poston, Daniel, Weisblum, Yiska, Hobbs, Alvaro, Bieniasz, Paul D. 2022. VPS29 Exerts Opposing Effects on Endocytic Viral Entry. In mBio, 13, e0300221. doi:10.1128/mbio.03002-21. https://pubmed.ncbi.nlm.nih.gov/35229640/
3. Parra Bravo, Celeste, Giani, Alice Maria, Madero-Perez, Jesus, Gong, Shiaoching, Gan, Li. 2024. Human iPSC 4R tauopathy model uncovers modifiers of tau propagation. In Cell, 187, 2446-2464.e22. doi:10.1016/j.cell.2024.03.015. https://pubmed.ncbi.nlm.nih.gov/38582079/
4. Healy, Michael D, McNally, Kerrie E, Butkovič, Rebeka, Collins, Brett M, Cullen, Peter J. . Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome. In Cell, 186, 2219-2237.e29. doi:10.1016/j.cell.2023.04.003. https://pubmed.ncbi.nlm.nih.gov/37172566/
5. Fuse, Atsuhito, Furuya, Norihiko, Kakuta, Soichiro, Saiki, Shinji, Hattori, Nobutaka. 2015. VPS29-VPS35 intermediate of retromer is stable and may be involved in the retromer complex assembly process. In FEBS letters, 589, 1430-6. doi:10.1016/j.febslet.2015.04.040. https://pubmed.ncbi.nlm.nih.gov/25937119/
6. Ye, Hui, Ojelade, Shamsideen A, Li-Kroeger, David, Bellen, Hugo J, Shulman, Joshua M. 2020. Retromer subunit, VPS29, regulates synaptic transmission and is required for endolysosomal function in the aging brain. In eLife, 9, . doi:10.7554/eLife.51977. https://pubmed.ncbi.nlm.nih.gov/32286230/
7. Yao, Y-Q, Lv, J-L. . Potential functions and causal associations of VPS29 in hepatocellular carcinoma: a bioinformatic and Mendelian randomization study. In European review for medical and pharmacological sciences, 27, 9586-9600. doi:10.26355/eurrev_202310_34131. https://pubmed.ncbi.nlm.nih.gov/37916325/
8. Bonifacino, Juan S, Hurley, James H. 2008. Retromer. In Current opinion in cell biology, 20, 427-36. doi:10.1016/j.ceb.2008.03.009. https://pubmed.ncbi.nlm.nih.gov/18472259/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen