C57BL/6JCya-Gfi1em1/Cya
Common Name:
Gfi1-KO
Product ID:
S-KO-18059
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gfi1-KO
Strain ID
KOCMP-14581-Gfi1-B6J-VB
Gene Name
Product ID
S-KO-18059
Gene Alias
Gfi-1; Pal-1; Pal1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gfi1em1/Cya mice (Catalog S-KO-18059) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000065478
NCBI RefSeq
NM_001267621
Target Region
Exon 2~7
Size of Effective Region
~6.9 kb
Detailed Document
Overview of Gene Research
Growth factor independence 1 (GFI1) is a DNA-binding zinc finger protein that acts as a transcriptional repressor [1]. It plays crucial roles in hematopoiesis, regulating the function of hematopoietic stem-and precursor cells, as well as differentiation along myeloid and lymphoid lineages [1]. GFI1 can recruit histone-modifying enzymes to target genes, thereby regulating gene expression [1]. It is also involved in the development of hair cells in the inner ear, regulating the expression of hair-cell-related genes [2].
In the context of acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS), GFI1 has been shown to be involved in their pathogenesis. For example, GFI1 expression levels correlate with patient survival and treatment response in both AML and MDS [1]. A non-synonymous single nucleotide polymorphism (SNP) of GFI1, GFI1-36N, is a prognostic factor for the development of AML and MDS. GFI1-36N leads to genome-wide epigenetic changes at GFI1-occupied target gene promoters and enhancers, and affects the response of leukemic cells to epigenetic drugs [1]. In addition, GFI1-36N expression is associated with increased chromosomal aberrations, mutational burden, and impaired homologous recombination-directed DNA repair in leukemic cells. Targeting related pathways can cause synthetic lethality in AML samples expressing GFI1-36N [3].
In conclusion, GFI1 is a key regulator in hematopoiesis and inner-ear development. In diseases like AML and MDS, studies on GFI1, especially through analysis of genetic variants like GFI1-36N, have provided insights into disease pathogenesis and potential therapeutic strategies. Understanding GFI1's functions helps in exploring new treatment options for hematological malignancies.
References:
1. Möröy, Tarik, Khandanpour, Cyrus. 2019. Role of GFI1 in Epigenetic Regulation of MDS and AML Pathogenesis: Mechanisms and Therapeutic Implications. In Frontiers in oncology, 9, 824. doi:10.3389/fonc.2019.00824. https://pubmed.ncbi.nlm.nih.gov/31508375/
2. Li, Zhuo, Chen, Hongzhi, Feng, Hao. 2025. Gfi1 in the inner ear: A retrospective review. In Developmental dynamics : an official publication of the American Association of Anatomists, , . doi:10.1002/dvdy.70019. https://pubmed.ncbi.nlm.nih.gov/40135914/
3. Frank, Daria, Patnana, Pradeep Kumar, Vorwerk, Jan, Jayavelu, Ashok Kumar, Khandanpour, Cyrus. . Germ line variant GFI1-36N affects DNA repair and sensitizes AML cells to DNA damage and repair therapy. In Blood, 142, 2175-2191. doi:10.1182/blood.2022015752. https://pubmed.ncbi.nlm.nih.gov/37756525/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen