C57BL/6JCya-Slc7a9em1/Cya
Common Name:
Slc7a9-KO
Product ID:
S-KO-18060
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc7a9-KO
Strain ID
KOCMP-30962-Slc7a9-B6J-VB
Gene Name
Product ID
S-KO-18060
Gene Alias
CSNU3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc7a9em1/Cya mice (Catalog S-KO-18060) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032703
NCBI RefSeq
NM_021291
Target Region
Exon 4~6
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Slc7a9, also known as the solute carrier family 7 member 9, is responsible for the exchange of dibasic amino acids and cystine (influx) for neutral amino acids (efflux). Cystine/cysteine transport by Slc7a9 is related to ferroptosis [1]. It is also one of the genes associated with cystinuria, a heritable disorder affecting the cysteine reabsorption by the renal system as well as the reabsorption of dibasic amino acids [2,4,5,6]. Genetic models, such as gene knockout mouse models, can be used to study its functions [3].
In gastric cancer, knockdown of Slc7a9 in cell lines and in patient-derived organoids (PDO), immunocompetent MFC mice allograft models and patient-derived xenograft (PDX) models suppressed cell proliferation, increased chemotherapy sensitivity, reduced cystine transport into cells, hampered intracellular cystine and GSH metabolic flow, decreased GSH synthesis, and increased lipid ROS levels, suggesting that Slc7a9 promotes gastric cancer progression by acting as a suppressor of ferroptosis [1]. In the Slc7a9-/-mouse model of cystinuria, the antioxidant l-Ergothioneine was able to prevent cystine lithiasis by increasing urinary cystine solubility and recovering renal GSH metabolism and mitochondrial function [3].
In conclusion, Slc7a9 plays a crucial role in cystine and amino acid transport, which is associated with ferroptosis and cystinuria. Studies using Slc7a9 knockout mouse models have revealed its functions in promoting cancer progression and in the pathogenesis of cystinuria, providing insights into potential therapeutic strategies for gastric cancer and cystinuria.
References:
1. Feng, Haoran, Yu, Junxian, Xu, Zhuoqing, Li, Yuan-Yuan, Liu, Bingya. 2024. SLC7A9 suppression increases chemosensitivity by inducing ferroptosis via the inhibition of cystine transport in gastric cancer. In EBioMedicine, 109, 105375. doi:10.1016/j.ebiom.2024.105375. https://pubmed.ncbi.nlm.nih.gov/39437660/
2. Halalsheh, Omar M, Al-Shehabat, Mustafa A, Al-Ghazo, Moh''D A, Al-Okour, Radwan, Altal, Omar. 2021. Analysis of SLC7A9 gene mutations among Jordanian patients with cystinuria. In Annals of medicine and surgery (2012), 63, 102182. doi:10.1016/j.amsu.2021.102182. https://pubmed.ncbi.nlm.nih.gov/33680451/
3. Mayayo-Vallverdú, Clara, López de Heredia, Miguel, Prat, Esther, Pallardó, Federico V, Nunes, Virginia. 2023. The antioxidant l-Ergothioneine prevents cystine lithiasis in the Slc7a9-/- mouse model of cystinuria. In Redox biology, 64, 102801. doi:10.1016/j.redox.2023.102801. https://pubmed.ncbi.nlm.nih.gov/37418888/
4. D'Ambrosio, Viola, Capolongo, Giovanna, Goldfarb, David, Gambaro, Giovanni, Ferraro, Pietro Manuel. 2021. Cystinuria: an update on pathophysiology, genetics, and clinical management. In Pediatric nephrology (Berlin, Germany), 37, 1705-1711. doi:10.1007/s00467-021-05342-y. https://pubmed.ncbi.nlm.nih.gov/34812923/
5. Breuning, M H, Hamdy, N A T C. . [From gene to disease; SLC3A1, SLC7A9 and cystinuria]. In Nederlands tijdschrift voor geneeskunde, 147, 245-7. doi:. https://pubmed.ncbi.nlm.nih.gov/12621979/
6. Mattoo, Aditya, Goldfarb, David S. . Cystinuria. In Seminars in nephrology, 28, 181-91. doi:10.1016/j.semnephrol.2008.01.011. https://pubmed.ncbi.nlm.nih.gov/18359399/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen