C57BL/6JCya-Tm6sf2em1/Cya
Common Name:
Tm6sf2-KO
Product ID:
S-KO-18062
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tm6sf2-KO
Strain ID
KOCMP-107770-Tm6sf2-B6J-VA
Gene Name
Product ID
S-KO-18062
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tm6sf2em1/Cya mice (Catalog S-KO-18062) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000110160
NCBI RefSeq
NM_001293795
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Tm6sf2, or Transmembrane 6 superfamily 2, is located on chromosome 19 (19p12) and its function is related to regulating plasma lipids. It is involved in lipid metabolism pathways, such as intestinal cholesterol absorption, hepatic cholesterol biosynthesis and transport, and the lipidation of very-low-density lipoproteins (VLDL) [1,5,6]. It has been recognized as a potential therapeutic target for cardiovascular disease and is associated with non-alcoholic fatty liver disease (NAFLD) [1,2]. Mouse models with overexpression or knockdown/knockout of Tm6sf2 have been crucial for in vivo validation of its role in regulating plasma lipid levels [1].
In gene-knockout studies, hepatocyte-specific Tm6sf2 knockout mice showed exacerbated tumour formation in metabolic dysfunction-associated steatotic liver disease-related hepatocellular carcinoma (MASLD-HCC) models, along with a reduction in interferon-gamma (IFN-γ)+CD8+ T cells, suggesting that Tm6sf2 can promote antitumour immunity via the NF-κB-IL-6 axis [3]. Myeloid cell-specific Tm6sf2 knockout mice on an ApoE-deficient background had inhibited atherosclerosis and decreased foam cells in plaques, with down-regulation of genes related to inflammation, cholesterol uptake, and endoplasmic reticulum stress [4]. Tm6sf2-/-rats and mice had higher hepatic triglyceride content, lower plasma cholesterol levels, and reduced lipid content in newly secreted VLDLs, indicating that Tm6sf2 is required for the lipidation of VLDL in the smooth endoplasmic reticulum [5].
In conclusion, Tm6sf2 is a key regulator in plasma lipid metabolism. Gene-knockout mouse models have been instrumental in revealing its role in diseases such as MASLD-HCC and atherosclerosis, highlighting its potential as a therapeutic target for these disease areas.
References:
1. Li, Ting-Ting, Li, Tao-Hua, Peng, Juan, Zheng, Xi-Long, Tang, Zhi-Han. 2017. TM6SF2: A novel target for plasma lipid regulation. In Atherosclerosis, 268, 170-176. doi:10.1016/j.atherosclerosis.2017.11.033. https://pubmed.ncbi.nlm.nih.gov/29232562/
2. Luo, Fei, Oldoni, Federico, Das, Avash. 2021. TM6SF2: A Novel Genetic Player in Nonalcoholic Fatty Liver and Cardiovascular Disease. In Hepatology communications, 6, 448-460. doi:10.1002/hep4.1822. https://pubmed.ncbi.nlm.nih.gov/34532996/
3. Zhang, Yating, Xie, Mingxu, Wen, Jun, Yu, Jun, Zhang, Xiang. 2025. Hepatic TM6SF2 activates antitumour immunity to suppress metabolic dysfunction-associated steatotic liver disease-related hepatocellular carcinoma and boosts immunotherapy. In Gut, 74, 639-651. doi:10.1136/gutjnl-2024-333154. https://pubmed.ncbi.nlm.nih.gov/39667906/
4. Zhu, Wenzhen, Liang, Wenying, Lu, Haocheng, Chen, Y Eugene, Guo, Yanhong. 2022. Myeloid TM6SF2 Deficiency Inhibits Atherosclerosis. In Cells, 11, . doi:10.3390/cells11182877. https://pubmed.ncbi.nlm.nih.gov/36139452/
5. Luo, Fei, Smagris, Eriks, Martin, Sarah A, Hobbs, Helen H, Cohen, Jonathan C. 2021. Hepatic TM6SF2 Is Required for Lipidation of VLDL in a Pre-Golgi Compartment in Mice and Rats. In Cellular and molecular gastroenterology and hepatology, 13, 879-899. doi:10.1016/j.jcmgh.2021.12.008. https://pubmed.ncbi.nlm.nih.gov/34923175/
6. Liu, Jing, Ginsberg, Henry N, Reyes-Soffer, Gissette. 2024. Basic and translational evidence supporting the role of TM6SF2 in VLDL metabolism. In Current opinion in lipidology, 35, 157-161. doi:10.1097/MOL.0000000000000930. https://pubmed.ncbi.nlm.nih.gov/38465912/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen