C57BL/6JCya-Trim5em1/Cya
Common Name:
Trim5-KO
Product ID:
S-KO-18075
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Trim5-KO
Strain ID
KOCMP-667823-Trim5-B6J-VA
Gene Name
Product ID
S-KO-18075
Gene Alias
EG667823; Gm8833
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trim5em1/Cya mice (Catalog S-KO-18075) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098179
NCBI RefSeq
XM_036153361
Target Region
Exon 1~4
Size of Effective Region
~12.9 kb
Detailed Document
Overview of Gene Research
TRIM5, a member of the tripartite motif protein family, is a restriction factor that defends mammalian cells against invading retroviruses like HIV-1 [1,2,4,7]. It recognizes and inactivates the capsid shell of incoming retroviral cores, coordinating the core uncoating and reverse transcription processes of the viral genome [1]. As an E3 ubiquitin ligase, TRIM5 also activates the TAK1 kinase, NF-κB and AP-1 signaling, and transcription of inflammatory cytokines and chemokines [2].
Cells lacking TRIM5 are unable to carry out PRKN-dependent and PRKN-independent mitophagy pathways, showing reduced mitochondrial function and uncontrolled immune activation in response to mitochondrial damage, indicating its role in mitophagy [5]. In hepatocellular carcinoma (HCC), mRNA and protein levels of TRIM5 are significantly upregulated, showing promising clinical prognostic value and strong association with immune cell infiltration [3]. TRIM5 also inhibits Senecavirus A replication by promoting the RIG-I-mediated type I interferon antiviral response [6].
In conclusion, TRIM5 plays essential roles in antiviral defense, mitophagy, and is associated with diseases such as HCC and viral infections. The study of TRIM5 knockout models has provided insights into its role in these biological processes and disease conditions, helping to understand the underlying mechanisms and potentially develop new therapeutic strategies.
References:
1. Ganser-Pornillos, Barbie K, Pornillos, Owen. 2019. Restriction of HIV-1 and other retroviruses by TRIM5. In Nature reviews. Microbiology, 17, 546-556. doi:10.1038/s41579-019-0225-2. https://pubmed.ncbi.nlm.nih.gov/31312031/
2. Grütter, Markus G, Luban, Jeremy. 2012. TRIM5 structure, HIV-1 capsid recognition, and innate immune signaling. In Current opinion in virology, 2, 142-50. doi:10.1016/j.coviro.2012.02.003. https://pubmed.ncbi.nlm.nih.gov/22482711/
3. Liu, Huanxiang, Tang, Yuhong, Zhang, Jiahao, Zhang, Chi, Bai, Dousheng. 2024. TRIM5 as a promising diagnostic biomarker of hepatocellular carcinoma: integrated analysis and experimental validation. In Functional & integrative genomics, 24, 63. doi:10.1007/s10142-024-01339-6. https://pubmed.ncbi.nlm.nih.gov/38517555/
4. de Silva, Suresh, Wu, Li. . TRIM5 acts as more than a retroviral restriction factor. In Viruses, 3, 1204-9. doi:10.3390/v3071204. https://pubmed.ncbi.nlm.nih.gov/21866272/
5. Saha, Bhaskar, Mandell, Michael A. 2022. The retroviral restriction factor TRIM5/TRIM5α regulates mitochondrial quality control. In Autophagy, 19, 372-373. doi:10.1080/15548627.2022.2084863. https://pubmed.ncbi.nlm.nih.gov/35659190/
6. Li, Huizi, Xie, Zhenxin, Lei, Xiaoling, Lin, Cunhao, Ning, Zhangyong. 2024. TRIM5 inhibits the replication of Senecavirus A by promoting the RIG-I-mediated type I interferon antiviral response. In Veterinary research, 55, 101. doi:10.1186/s13567-024-01354-2. https://pubmed.ncbi.nlm.nih.gov/39143491/
7. Sokolskaja, Elena, Luban, Jeremy. 2006. Cyclophilin, TRIM5, and innate immunity to HIV-1. In Current opinion in microbiology, 9, 404-8. doi:. https://pubmed.ncbi.nlm.nih.gov/16815734/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen