C57BL/6JCya-Zdhhc7em1/Cya
Common Name:
Zdhhc7-KO
Product ID:
S-KO-18085
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Zdhhc7-KO
Strain ID
KOCMP-102193-Zdhhc7-B6J-VB
Gene Name
Product ID
S-KO-18085
Gene Alias
Gramp2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zdhhc7em1/Cya mice (Catalog S-KO-18085) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034280
NCBI RefSeq
NM_133967.3
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Zdhhc7, also known as zinc finger DHHC-type palmitoyl transferase 7, is a palmitoyltransferase that plays a crucial role in protein S-palmitoylation. This post-translational modification is involved in various biological pathways, such as inflammasome activation, autophagy, androgen receptor regulation, and T-helper cell differentiation [1-4]. It is important for normal physiological functions and is associated with multiple disease conditions including chronic inflammatory diseases, prostate cancer, and inflammatory bowel disease. Mouse models with Zdhhc7 knockout (KO) or conditional knockout (CKO) have been instrumental in understanding its function in vivo [1-6].
In macrophages, Zdhhc7 knockout reduces NLRP3-mediated inflammasome activation as it is responsible for palmitoylating NLRP3 Cys126, which is essential for NLRP3 activation [1]. In ATG16L1-KO HeLa cells, re-expressing wild-type ATG16L1 but not the S-palmitoylation-deficient mutant rescues the defect in LC3 lipidation and autophagosome formation, indicating that Zdhhc7-mediated S-palmitoylation of ATG16L1 is crucial for autophagy [2]. In prostate cancer cells, Zdhhc7 depletion increases oncogenic properties, while restoring Zdhhc7 suppresses cell proliferation and invasion in vitro and tumor growth in vivo, as it inhibits androgen receptor gene transcription [3]. In a mouse model of inflammatory bowel disease, knockout of Zdhhc7 relieves symptoms, suggesting its role in promoting TH17 cell differentiation through STAT3 palmitoylation [4].
In conclusion, Zdhhc7 is essential for multiple biological processes through its role in protein S-palmitoylation. KO/CKO mouse models have been key in revealing its functions in diseases such as chronic inflammatory diseases, cancer, and inflammatory bowel disease. Understanding Zdhhc7 provides potential therapeutic strategies for these related disease conditions.
References:
1. Yu, Tao, Hou, Dan, Zhao, Jiaqi, Linder, Maurine E, Lin, Hening. 2024. NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation. In Cell reports, 43, 114070. doi:10.1016/j.celrep.2024.114070. https://pubmed.ncbi.nlm.nih.gov/38583156/
2. Wei, Fujing, Wang, Yu, Yao, Jia, Kong, Eryan, Yang, Aimin. 2024. ZDHHC7-mediated S-palmitoylation of ATG16L1 facilitates LC3 lipidation and autophagosome formation. In Autophagy, 20, 2719-2737. doi:10.1080/15548627.2024.2386915. https://pubmed.ncbi.nlm.nih.gov/39087410/
3. Lin, Zhuoyuan, Agarwal, Shivani, Tan, Song, Zhao, Jonathan C, Yu, Jindan. 2023. Palmitoyl acyltransferase ZDHHC7 inhibits androgen receptor and suppresses prostate cancer. In Oncogene, 42, 2126-2138. doi:10.1038/s41388-023-02718-2. https://pubmed.ncbi.nlm.nih.gov/37198397/
4. Zhang, Mingming, Zhou, Lixing, Xu, Yuejie, Linder, Maurine E, Lin, Hening. 2020. A STAT3 palmitoylation cycle promotes TH17 differentiation and colitis. In Nature, 586, 434-439. doi:10.1038/s41586-020-2799-2. https://pubmed.ncbi.nlm.nih.gov/33029007/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen