C57BL/6JCya-Tent5aem1/Cya
Common Name:
Tent5a-KO
Product ID:
S-KO-18099
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tent5a-KO
Strain ID
KOCMP-212943-Tent5a-B6J-VA
Gene Name
Product ID
S-KO-18099
Gene Alias
BAP014; D930050G01Rik; Fam46a
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tent5aem1/Cya mice (Catalog S-KO-18099) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034802
NCBI RefSeq
NM_001160378
Target Region
Exon 1~3
Size of Effective Region
~4.5 kb
Detailed Document
Overview of Gene Research
Tent5a, also known as FAM46A, is a cytoplasmic poly(A) polymerase. It is involved in non-canonical polyadenylation processes, which play crucial roles in regulating gene expression at the post-transcriptional level. These processes are essential for various biological functions, including the production of secreted proteins and the regulation of mRNA stability [2,3].
In a Tent5a knockout (KO) mouse line, there are defects in bone formation. The KO mice display bone fragility and skeletal hypomineralization due to quantitative and qualitative collagen defects, as Tent5a is responsible for the cytoplasmic polyadenylation of mRNAs encoding Col1α1, Col1α2, and other secreted proteins involved in osteogenesis [2].
In another study, knockdown of Tent5a in C2C12 cells (a myoblast cell line) inhibited cell proliferation, migration, and the maturation of type I muscle fibers, both in vitro and in vivo. This was associated with decreased myogenin expression, suggesting Tent5a's role in muscle fiber formation in the context of adolescent idiopathic scoliosis [1].
In conclusion, Tent5a is essential for proper bone formation and muscle fiber maturation. The use of Tent5a KO mouse models has revealed its critical functions in these biological processes. These findings contribute to our understanding of the molecular mechanisms underlying bone-related diseases and adolescent idiopathic scoliosis.
References:
1. Luo, Ming, Yang, Huiliang, Wu, Diwei, Huang, Shishu, Song, Yueming. 2022. Tent5a modulates muscle fiber formation in adolescent idiopathic scoliosis via maintenance of myogenin expression. In Cell proliferation, 55, e13183. doi:10.1111/cpr.13183. https://pubmed.ncbi.nlm.nih.gov/35137485/
2. Gewartowska, Olga, Aranaz-Novaliches, Goretti, Krawczyk, Paweł S, Prochazka, Jan, Dziembowski, Andrzej. . Cytoplasmic polyadenylation by TENT5A is required for proper bone formation. In Cell reports, 35, 109015. doi:10.1016/j.celrep.2021.109015. https://pubmed.ncbi.nlm.nih.gov/33882302/
3. Menezes, Miriam R, Balzeau, Julien, Hagan, John P. 2018. 3' RNA Uridylation in Epitranscriptomics, Gene Regulation, and Disease. In Frontiers in molecular biosciences, 5, 61. doi:10.3389/fmolb.2018.00061. https://pubmed.ncbi.nlm.nih.gov/30057901/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen