C57BL/6JCya-Irs1em1/Cya
Common Name
Irs1-KO
Product ID
S-KO-18106
Backgroud
C57BL/6JCya
Strain ID
KOCMP-16367-Irs1-B6J-VA
When using this mouse strain in a publication, please cite “Irs1-KO Mouse (Catalog S-KO-18106) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Irs1-KO
Strain ID
KOCMP-16367-Irs1-B6J-VA
Gene Name
Product ID
S-KO-18106
Gene Alias
G972R, IRS-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000069799
NCBI RefSeq
NM_010570
Target Region
Exon 1
Size of Effective Region
~4.6 kb
Overview of Gene Research
Irs1, or Insulin Receptor Substrate 1, is a crucial signal transduction adapter. It facilitates extracellular signal transduction from insulin-like growth factor-1 receptor and its family members to downstream proteins. IRS1 is involved in the insulin signaling pathway, which is essential for the hormonal control of metabolism [5]. Mutations in this gene have been associated with an increased ability to develop type 2 diabetes, highlighting its importance in metabolic health [4].
In cancer research, IRS1 has been found to play diverse roles. In thyroid cancer, it is highly expressed, promoting metastasis through epithelial-mesenchymal transition (EMT) and the PI3K/AKT pathway, suggesting it could be a stratified marker and therapeutic target [1]. In oral squamous cell carcinoma (OSCC), IRS1 is also highly expressed, and its silencing decreases cell proliferation, increases apoptosis, and hinders EMT through the p53/Line-1 signaling pathway [2]. In ovarian cancer, repressing IRS1/2 by NT157 inhibits malignant behaviors by inactivating the PI3K/AKT/mTOR pathway and inducing autophagy [3]. In esophageal squamous cell carcinoma, IRS1 expression is associated with lymph node metastases and prognosis, with overexpression negatively associated with metastasis [6].
In conclusion, Irs1 is essential for insulin-mediated signal transduction and metabolism. Its role in various cancers, as revealed through functional studies, shows its potential as a biomarker and therapeutic target in oncology. The study of Irs1 knockout or conditional knockout mouse models could further clarify its functions in normal and disease-related biological processes.
References：
1. Yu, Fang, Huang, Dongdong, Kuang, Yeye, Zhou, Jie, Teng, Xiaodong. 2024. IRS1 promotes thyroid cancer metastasis through EMT and PI3K/AKT pathways. In Clinical endocrinology, 100, 284-293. doi:10.1111/cen.15005. https://pubmed.ncbi.nlm.nih.gov/38172081/
2. Xiao, Yanbo, Zhu, Xuan, Li, Qun, Liu, Xun, Tan, Jin. . m6A-Mediated IRS1 Regulates the Development of Oral Squamous Cell Carcinoma through p53/Line-1 Signaling. In Frontiers in bioscience (Landmark edition), 29, 257. doi:10.31083/j.fbl2907257. https://pubmed.ncbi.nlm.nih.gov/39082352/
3. Li, Cai-Xia, Men, Chuan-Di, Yang, Wei-Hong, Zhu, Ji-Hui, Cheng, Zhong-Ping. 2023. Repressing IRS1/2 by NT157 inhibits the malignant behaviors of ovarian cancer through inactivating PI3K/AKT/mTOR pathway and inducing autophagy. In The Kaohsiung journal of medical sciences, 39, 377-389. doi:10.1002/kjm2.12652. https://pubmed.ncbi.nlm.nih.gov/36727938/
4. Keshavarzi, Fatemeh, Golsheh, Shadi. 2019. IRS1- rs10498210 G/A and CCR5-59029 A/G polymorphisms in patients with type 2 diabetes in Kurdistan. In Molecular genetics & genomic medicine, 7, e631. doi:10.1002/mgg3.631. https://pubmed.ncbi.nlm.nih.gov/30884193/
5. Copps, K D, White, M F. 2012. Regulation of insulin sensitivity by serine/threonine phosphorylation of insulin receptor substrate proteins IRS1 and IRS2. In Diabetologia, 55, 2565-2582. doi:10.1007/s00125-012-2644-8. https://pubmed.ncbi.nlm.nih.gov/22869320/
6. Lei, Yufei, Jamal, Muhammad, Zeng, Xingruo, Xie, Songping, Zhang, Qiuping. 2022. Insulin receptor substrate 1(IRS1) is related with lymph node metastases and prognosis in esophageal squamous cell carcinoma. In Gene, 835, 146651. doi:10.1016/j.gene.2022.146651. https://pubmed.ncbi.nlm.nih.gov/35688292/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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